基于局域表面等离子体共振的联用检测仪器具有所需装置小、光路简单、易于集成等优点，有望发展成为一种新型的便携化多功能分析检测仪器，从而受到人们的广泛关注和重视。但迄今为止，有关此类联用检测技术的国内外研究仍很缺乏，仅处于初期探索阶段。本项目基于纳米光子学理论和非常规纳米加工技术可控制备具有高灵敏局域表面等离子体共振效应的三维金纳米有序阵列基底；以三维金纳米有序阵列基底-微流控芯片为核心部件，整合局域表面等离子体传感和电化学石英晶体谐振传感技术，开发一种新型的多功能表面分析检测平台，用于监测传感界面的折射率、质量和电信号变化等多个参数；系统研究以重金属离子纳米探针为基础的免疫竞争法快速检测过程，以及探索重金属离子在电极表面近程扩散的规律，为我国研制具有自主知识产权的便携化光学或电化学检测装置提供实验依据和理论基础，相关成果可应用于疾病监控、食品卫生安全和环境检测等领域。

Localized surface plasmon resonance based joint-detection instrument has attracted wide attention, because the instrument only requires small devices, simple optical path, and is easy to be integrated with other instruments, which make the joint-detection instrument possible to become a new type of portable analytical instrument for multifunctional detection. But so far, the research on this kind of joint-detection technology is still at an early stage. Here, we fabricate a three-dimensional gold nanoarray substrate with high refractive index sensitivity based on the nanophotonics theory and unconventional nanofabrication technique, bond the substrate with a microfluidic chip to be a core component, integrate localized surface plasmon resonance with electrochemical quartz crystal microbalance sensor, to develop a new type of surface analysis platform for monitoring the changes in refractive index, mass and electrochemical signals etc. at the sensing interface. We also systematically study the nanoprobes based competitive immunoassay test, as well as the short-range diffusion process of heavy metal ions near the surface. This project will provide new experimental and theoretical data, which will be useful for the development of portable optical/electrochemical detection devices with China’s independent intellectual property rights, and the research findings would have great potential applications in the field of disease control, food safety and environmental testing etc.