新类型RNA的系统识别和生物学功能研究是RNA研究最重要内容之一。细胞表面RNA(cell surface-bound RNA)的存在已经得到证实，但是对于细胞表面RNA缺少深入系统研究。本研究将在我们已有研究基础上，以肿瘤细胞为模型，对肿瘤细胞表面结合RNA进行高通量RNA测序、验证和生物信息学分析，获得典型的肿瘤细胞系的细胞表面RNA表达谱，发现其表达模式；探讨其在不同的干预条件下RNA分子的完整性、结合状态和递呈变化，阐释细胞表面RNA加工生成机制；进一步选择典型的特定细胞表面RNA分子（线粒体RNA/mRNA/lncRNA等），分析其对细胞增殖、克隆形成、细胞周期、细胞凋亡、侵袭转移能力等细胞生物学功能的影响，进而揭示肿瘤细胞表面RNA的生物学功能。通过基于肿瘤细胞表面RNA研究所获得的发现，将从一个全新的角度揭示RNA生物学功能的多样性，为肿瘤的研究和治疗提供新的技术和思路。

System identification on a new type of RNA and research on its biological function is one of the most important research areas about RNA. Cell surface-bound RNA has been confirmed, however, the scientists don't understand cell surface-bound RNA systematically and deeply. In this study, based on our previous studies, the first, we will identify cell surface-bound RNAs in different cancer cell line models by high-throughut RNA-sequencing and obtain the typical tumor cell line expression profile of cell surface-bound RNA, and we also found the expression pattern of cancer cell surface-bound RNA by bioinformatics analysis. The second, we will investigate the molecule integrity, binding status and presentation on the cell surface of cell surface-bound RNA and preliminary illuminate the biogenesis mechanism of cell surface-bound RNA. The third, further selection of the typical cell surface-bound RNAs(e.g. mitochondrial RNA/mRNA/lncRNA, etc.), we will study their effects on the cell proliferation, cell clone formation, cell cycle, cell apoptosis, cell invasion and metastasis and then reveal the biological function of cancer cell surface-bound RNA. As a result of our research findings based on the tumor cell surface-bound RNA, we will be from a new perspective to reveal the diversity of the biological functions of RNA and these research findings will also provide new technologies and ideas for cancer research and treatment.