采用溶液培养及LC-MS/MS与现代分子技术，从根寄生植物向日葵列当(O. cumana)的特定寄主作物向日葵不同抗性品种根系分泌物中提取天然的根寄生植物的各种萌发刺激物和吸器诱导物，进行化学组成与结构鉴定及应用效果研究；明确上述不同识别信号物质(SL、DCL等)及其协同作用，及与人工合成萌发刺激物、吸器诱导物质在寄生植物与寄主识别中的种子萌发、诱导吸器的异同性及其机理；探明不同类型与水平的氮磷钾营养与组合配比，及其与植物内源激素、除草剂的协同调控作用，实现对识别信号物质的营养与化学调控；克隆O. cumana中参与SL信号感受的关键基因（MAX2等），并对相应基因进行功能分析。从而揭示化学信号物质在寄生植物和寄主识别中的相互作用及其机理，加深对植物间和根际中化学物质与分子信息传递的了解，筛选有效的、环境友好的化学信号物质并用于根寄生杂草“自杀性萌发”等防控，具有重要的科学意义。

Using hydroponical culture and modern analytical techniques such as LC-MS/MS, this project is to extract and identify the natural germination stimulants and haustorium inducing factors of root parasitic plant (Orobanche) from various cultivars of sunflowers with different resistant capabilities; This experiment also intends to understand the mechanism of natural germination stimulants (such as SL and DCL), synthetic germination stimulants as well as haustorium inducing factors on seed germination, growth and development of Orobanche plant in the host recognition; The present study attempts to use various nutrients (N, P and K), along with endogenous plant hormones and herbicides to regulate signaling factors in sunflower and clone SL-receptor genes (such as MAX2) in O. cumana for function analysis. This research could better understand the molecular mechanism of chemical signals (germination stimulants and plant hormones etc) in the host recognition of root parasitic plant growth and development, through the selection of effective and environmental friendly germination stimulants and their possible application for “suicidal germination” of root parasitic weeds. Thus, the present project is of great importance in the understanding of host recognition of root parasitic weeds.