聚合物表面微结构调控及其对蛋白质、细胞微观粘附行为的影响规律

材料表面微结构影响蛋白质和细胞在其表面的粘附行为，但材料表面调控蛋白质和细胞粘附行为的分子机制以及蛋白质和细胞的微观粘附机理至今仍不清楚。本项目拟采用单根聚合物微、纳米纤维作为模型材料，构建蛋白质、细胞与材料之间的粘附模型。主要以可降解聚酯和苯乙烯类聚合物为原料，聚合物纤维通过电纺丝方法制备，通过改变表面拓扑结构、引入化学基团和生物分子三种方法调控表面微结构。利用原子力显微镜直接测定蛋白质、细胞在纤维表面的粘附力、粘附能，把材料表面的微结构调控和粘附行为的定量分析结合起来，系统研究蛋白质、细胞在纤维表面的微观粘附行为；同时以聚合物膜为对比材料，进而揭示材料表面微结构调控蛋白质、细胞在其表面粘附行为的分子机制。本项目不但有利于建立蛋白质、细胞微观粘附行为的研究方法，而且有利于认识蛋白质、细胞的粘附本质，为设计智能粘附和定向粘附的材料表面提供理论指导和实践支持。

The microstructure of the material surface influences the adhesive behavoir of protein/cell on the surface. However, the mechanism of adhesion of protein/cell and control of microstructure on adhesion are unclear until now. In this proposal we choose a single micro/nanofiber as a model to study the adhesion between fiber and protein/cell. The nano/microfibers of degradable polyester and styrenic copolymer are fabricated by electrospinning technique and the microstructure is controlled by adjusting surface topological structure, introduction of chemical groups and biomacromolecules. The adhesion and adhesion energy are directly measured by atomic force microscope to investigate the adhesive behavior at the molecular level. In addition, the adhesion between polymer film and protein/cell is compared with that between micro/nanofiber and protein/cell, which facilitates establishing the relationship between microstructure and adhesion. This proposal will not only pave the way to study the adhesion between protein/cell and materials to explore the nature of adhesion, but also provide theoretical and practical guidance for designing the surface with smart and oriented adhesion.