抗溶血高分子材料表面结构构建及其抗红细胞溶血机理

高分子材料与红细胞长期接触，不可避免地造成红细胞溶血，引发体内多种疾病。长期接触造成溶血的本质是红细胞膜的氧化损伤，但传统的高分子材料本体和表面改性方法无法抑制红细胞膜氧化。本项目提出了一种全新的抗红细胞溶血策略，即在基体高分子材料表面构建具有生物分子释放功能的三维多级结构。与红细胞接触过程中，生物分子释放出来与红细胞作用，从根本上抑制红细胞的氧化损伤。本项目拟以聚烯烃与苯乙烯类热塑性弹性体为基体材料，以红细胞存储材料为研究体系，系统研究材料表面接枝、三维多级结构构建、生物分子释放及其与红细胞相互作用、新型材料的长期抗溶血功能。揭示红细胞在存储过程中的氧化过程和溶血机理，阐明材料的长期抗红细胞溶血机理，建立降低溶血的有效方法，进而设计和制备新型抗溶血材料。本项目不仅解决红细胞体外存储过程中的溶血问题，而且将为解决植入类材料的溶血问题提供理论指导和技术支持。

Long-term contact of polymer materials with red blood cells (RBCs) inevitably causes the hemolysis, leading to an intrinsic mechanism for human disease. However, the conventional methods based on bulk and surface modification of substrate polymer have slight effects on hemolysis, because hemolysis is resulted from the oxidative damage of RBC membrane. In this project, a new strategy based on the controlled release of bio-molecules from the blood-contacting surface to decrease the oxidative injure of RBCs is proposed. The polyolefin and polystyrene-based thermoplastic elastomers will be used as substrates, and novel anti-hemolytic polymer for RBCs preservation as the model system. The surface modification, 3D architecture construction, release of bio-molecules and their interactions with RBCs, and long-term anti-hemolytic capability of novel polymer materials will be systematically investigated. As a result, the interactions between functional surfaces and RBCs, hemolytic mechanism of RBCs, anti-hemolytic mechanism of new materials, and method for decreasing hemolysis will be established. In addition, the new polymer materials with anti-hemolytic capability will be developed. The project will not only pave new way to fabricate high hemocompatible biomaterials for RBC storage in vitro, but provide basic principles to design and develop anti-hemolytic biomaterials for implantation in vivo.