近年来研究发现，纤维二糖可以稳定诱导粗糙脉孢菌纤维素酶表达，但诱导表达信号传导途径的核心基因元件大部分没有被克隆鉴定。近期我们发现在葡萄糖苷酶突变体背景下，进一步缺失一个新的蛋白（命名为CLP1蛋白）能够显著提高纤维二糖对纤维素酶的诱导。本申请将重点针对CLP1蛋白，利用粗糙脉孢菌完备的遗传操作系统，结合前期构建的纤维二糖稳定诱导纤维素酶体系，全面解析CLP1蛋白在纤维二糖诱导纤维素酶表达信号传导通路中的分子功能。将构建CLP1与该通路已知元件葡萄糖苷酶和纤维二糖转运蛋白组合突变体，分析这一系列突变体的纤维素酶诱导表达状况，从而解析CLP1与葡萄糖苷酶、纤维二糖转运蛋白CDT1、CDT2在这一过程中的相互作用等。CLP1蛋白，作为粗糙脉孢菌纤维素酶诱导表达信号传导通路中新元件，其分子功能的解析，对阐明整个纤维素酶信号传导途径的分子机理和提高纤维素酶工业菌种发酵水平都有重要意义。

The cellulase signal transduction is still far from completely understood in filamentous fungi.Recently research on Neurospora crassa shown the cellobiose can induce the cellulase expression when two or three major b-glucosidases were deleted.Based on we have constructed the mutant of bg13 and bg123 system, in which the cellulases can be inducted efficiently and reproducibly by cellobiose. We found a novel protein(named as CLP1) has critical roles in the cellulases induction pathway, the deletion of CLP1 in bg123 background, the cellulases induction were significantly increased in both cellobiose and cellulose condition. Based on this finding, in this project,we will focus on functional characterization of CLP1 protein,including the roles of CLP1 in cellulses induction pathway, and the interactions between the CLP1 and the other known proteins of the pathways such as b-glucosidases and cellodextrin transporters.The characterization of CLP1 would definitely shed novle light on the mechanism of cellulases induction pathway and would also give the helps for industial cellulases production strain improvement.