模式生物中已经证实泛素蛋白酶体途径（UPS）在顶体反应和精卵融合过程中具有的重要作用。泛素化调节决定精子膜蛋白、顶体反应和精卵相互作用过程中关键蛋白的活性，因此关注泛素蛋白酶体途径在中华绒螯蟹生殖中的作用对我们进一步理解河蟹生殖机制具有重要意义。河蟹精子多精着卵、单精受精，以及体内着精、体外受精和群体生殖洄游的特点，使其成为研究生殖的最佳模型。基于河蟹精巢和副性腺转录组数据，从中发现291个泛素蛋白和49个蛋白酶体亚基基因，为全面了解生殖相关泛素蛋白酶体途径提供了基础数据，利用完善的泛素研究体系研究河蟹精子顶体反应和精卵相互作用过程中泛素蛋白酶体调节方式，重点关注精子受体蛋白、精子头粒蛋白、精子顶体蛋白、受精素、Prohibitin等在精子中通过UPS调控实现功能调节的蛋白，利用UPS途径专一性抑制剂处理精子分析上述蛋白在精卵相互作用过程中的功能，为阐明UPS在河蟹生殖中的功能积累分子证据

Ubiquitin Proteasome System (UPS) play important role in sperm maturation, acrosome reaction (AR), sperm-oocyto interaction during fertilization. Ubiquitination regulate the key proteins function in the sperm membrane, AR and sperm zona penetration, so it is important to focus on the function of UPS in reproduction of Chinese mitten crab, Eriocheir sinensis. Chinese mitten crab has the character of reproductive migration and sperm is multi-sperm interaction with one spawn but monospermy ultimately, sperm adheren spawn in vivo but fertiliztion in vitro. The special character of reproduction in mitten crab made them a good model for fertilization study. We based on the transcriptome of testis and ASG from E. sinensis identified 291 unigenes related to ubiquitin and 49 unigenes related to proteasom,that intrest us to study the function of UPS in sperm acrosome reaction in E. sinensis. We will use the well-established research system on UPS in mammal, such as the general antibody and all kinds of activators and inhibitors. Especially pay attention to the UPS regulation on key proteins, such as Vitelline coat protein, Acrosin, Sperm acrosomal protein, Fertilin, Prohibitin in sperm in E. sinensis. Applying RACE,Western Blotting and immunolocalization methods to analysis the regulation mechanism of UPS in capacitation and acrosome reaction in E.sinensis, that will accumulate molecular evidence to elucidate the mechnism of male reproduction of Chinese mitten crab.