酵母利用木质纤维素水解液发酵产微生物油脂可为生物柴油的制备提供廉价、来源广泛、可再生的油脂原料。该技术仍需大量多因素、多水平的研究以满足工业应用要求，但目前微生物油脂含量和油脂组成复杂繁琐的分析方法降低了研究的效率，因此开发新型的分析方法十分重要。本项目以可高效利用木质纤维素水解液发酵的新型油脂酵母Trichosporon dermatis CH007为研究对象，通过揭示荧光染料荧光强度与油脂含量、油脂组成的关系及染料进入酵母细胞和荧光稳定性等规律，阐明细胞油脂含量和油脂组成的细胞荧光分析机理，为建立其分析方法提供理论基础；筛选获得荧光强度与细胞油脂含量、油脂组成具有显著相关性的荧光染料，然后使用响应面分析获得最优的细胞荧光分析方法，并在实际发酵中评价该方法的适应性、准确性和稳定性，最终建立高效、准确、简便的油脂含量和油脂组成的细胞荧光分析方法，有效提高油脂发酵技术的研究效率。

Microbial oil production by fermentation of oleaginous yeasts on lignocellulosic hydrolysates can offer low-cost, great available, and renewable lipid feedstock for biodiesel production. This technology still needs great amount of research with various factors and levels to satisfy the requirement of industrial application, but the complex and trivial analytical method of lipid content and lipid composition reduce the efficiency of the research on this technology, thus it is important to explore new kind of analytical method. In this study, new oleaginous yeast Trichosporon dermatis CH007 which can utilize lignocellulosic hydrolysates efficiently for fermentation is chosen as research object. By revealing the relationship between fluorescent intensity of fluorescent dyes and lipid content / lipid composition, and the laws of fluorescent dyes’ entering into yeast cells, fluorescent stability, etc., the cell fluorescent analytical mechanism of cellular lipid content and lipid composition can be elucidated, this can offer theoretical basis for the establishing its analytical method. In addition, fluorescent dyes whose fluorescent intensity related significantly to cellular lipid content / lipid composition will be screened, then the optimal cell fluorescent analytical method will be obtained by response surface analysis, and the adaptability, accuracy and stability of this method will be evaluated in the actual fermentation. Finally, efficient, accurate and convenient cell fluorescent analytical method for lipid content and lipid composition measurement will be built, and this will increase the research efficiency of lipid fermentation technology effectively.