弓形虫慢性感染可造成宿主神经精神系统的紊乱，特别是导致神经递质多巴胺的失调，而这一过程是通过改变miR-132实现的，但是其具体作用机制尚不明确。我们的前期研究发现lncRNA LNC_000959 与miR-132存在结合位点，并且表达量与miR-132存在相关性，而miR-132又可调控与多巴胺分泌有关的Nurr1基因的表达。由此我们推测LncRNA LNC_000959可作为 miR-132 的 ceRNA 调控 Nurr1的表达从而影响慢性感染弓形虫后小鼠大脑的多巴胺的正常水平。本研究拟开展三方面研究：（1）LncRNA LNC_000959对感染小鼠脑内多巴胺水平的影响；（2）LncRNA LNC_000959、miR-132及 Nurr1 之间的分子作用机制；（3）LncRNA LNC_000959 竞争性结合 miR-132 调控 Nurr1 对感染小鼠脑内多巴胺水平的影响。

The chronic infection rate of Toxoplasma gondii is very high in humans, which can cause disturbances and behavioral changes in the host neuropsychiatric system, posing a serious threat to human health. Toxoplasma infection can cause brain damage, changes in synapses, especially the neurotransmitter dopamine, and this process is achieved by altering miR-132, but its specific mechanism of action is still unclear. Our previous study found that lncRNA LNC_000959 has a binding site with miR-132, and the expression level is related to miR-132, and miR-132 can regulate the expression of Nurr1 gene related to dopamine secretion. Thus, we hypothesized that LncRNA LNC_000959 can act as a ceRNA of miR-132 to regulate the expression of Nurr1 and affect the normal level and function of dopamine in the brain of mice infected with T.gondii. This study is intended to conduct three studies: (1) the effect of LncRNA LNC_000959 on dopamine levels in infected mice; (2) the molecular mechanism of action between LncRNA LNC_000959, miR-132 and Nurr1; (3) the competitive binding of LncRNA LNC_000959 miR-132 regulates the effect of Nurr1 on dopamine levels in infected mice. It is hoped that through these efforts to find therapeutic targets for neuropsychiatric disorders induced by chronic infection of Toxoplasma gondii, it provides a powerful theoretical and experimental basis for the diagnosis and treatment of clinical chronic toxoplasmosis.