糖尿病神网膜神经退行性变（DRN）所致视功能损伤是重大的医疗和社会问题，尚无有效治疗，根据病变特点，预防DRN可能更为有效和关键。白藜芦醇是广泛存于植物中的多酚，因其明确的神经保护作用，一直是研究的热点，但目前其研究结论主要基于动物和细胞实验，所用剂量大，在人体难以实现，低浓度白藜芦醇作用及机制不明。我们在前期研究中也发现：眼科手术患者口服白藜芦醇后眼组织中白藜芦醇的浓度近千倍低于实验室所用浓度。2015年nature文献填补了低浓度白藜芦醇作用及机制的空白：通过激活TyrRS/PARP通路,启动应激反应，上调生存基因的表达。本项目拟在前期研究基础上，通过细胞和动物实验探寻白藜芦醇在眼组织分布，证实低剂量白藜芦醇对视网膜神经的保护作用，运用代谢组学，分生等技术探寻TyrRS/PARP通路的激活及下游相关物质的表达。大医治未病，本研究有望为预防和早期治疗DRN提供实验和理论依据。

Visual function impairment related to diabetic retinal neurodegeneration (DRN) comes to be a medical and social problem while there is no effective treatment by far.According to its characteristics,prevention of DRN may be more effective and critical.Resveratrol is a small polyphenol found in various plants.A number of studies have concentrated on its role of neuro-protective effect which is based mainly on cell and animal experiments.However, its doses applied in animal and cell experiments are too large to be suit for human,therefore its effect and mechanism in low dose remains unknown.In our previous study,patients undergoing eye surgery received oral administration of resveratrol , then we detected the concentration of resveratrol and its metabolites in eye tissues (conjunctiva, aqueous humor and vitreous humor) ,which was nearly 1000 times lower than that in most cell and animal experiments.In 2015,a study published in Nature revealed a new mechanism of low-dose resveratrol：by activing the TyrRS/PARP1 pathway and stress response,and upregulating the expression of survival genes. This project is proposed on the basis of our previous studies,we are planning to detect the concentration of resveratrol in eye and identify the neuro-protective effect of low-dose resveratrol by animal and cell experiments,and to work out its mechanism and the role of TyrRS/PARP pathway by metabolomics and molecular biology techniques.Preventive treatment beats the best cure.This study may provide a new idea for the prevention of DRN.