条锈病是小麦生产上危害最重的真菌病害之一，合理利用抗病品种是防治小麦条锈病最为有效、经济、环保的措施。然而，小麦品种常因条锈菌的频繁变异而“丧失”抗性，失去利用价值。因而，深入开展小麦抗条锈病机理研究，对开发条锈病防控新策略具有重要意义。在前期研究中，通过RNAi技术鉴定获得了小麦感条锈病基因TaSTK蛋白激酶，酵母双杂交筛选获得了其5个潜在的互作靶标，其中1个为条锈菌分泌蛋白，4个为小麦抗病性相关基因。为此，本项目拟在前期基础上，解析条锈菌分泌蛋白是如何调控小麦TaSTK蛋白激酶抑制寄主的免疫反应，以及TaSTK蛋白激酶如何作用于寄主的抗病靶标基因，以期揭示TaSTK蛋白激酶介导的小麦感条锈病机理，明确TaSTK基因的调控网络，并利用基因编辑技术创制新型抗条锈病材料，为小麦抗条锈病的遗传改良提供资源和技术支撑，为开发新的病害防控策略提供借鉴。

Wheat stripe rust caused by Puccinia striiformis f. sp tritici (Pst) is one of the most severe devastating diseases on wheat, which causes serious damage. Planting resistant cultivars is the most effective and friendly way to control this disease. However, the rapid virulence variation of Pst leads to a loss of resistance in currently resistant wheat cultivars, which results in frequent disease epidemics. Therefore, research on the mechanism underlying wheat resistance to Pst is of great significance in developing novel strategy to control the disease. In the preliminary study, a wheat serine-thronine protein kinase TaSTK is responsible for the susceptibility of wheat to Pst by RNAi technology. Yeast two hybrid screening revealed that one secreted protein from Pst and 4 wheat defense related protein could be targeted by TaSTK. Thereby, in this study, we plan to further analyze the manner in which the Pst secreted protein target TaSTK to suppress host immunity, and demonstrate the modulation of TaSTK on the following host targets. The results will reveal the regulation mechanism underlying TaSTK on wheat susceptibility to Pst and clarify the modulation network of TaSTK. Furthermore, we aim to create new resistance materials by genome editing TaSTK, which will provide technical support for genetic improvement of wheat resistance to stripe rust, and lay foundations to develop new strategies for durable and efficient control of stripe rust disease.