包埋缓释载体的共性问题是难以同时赋予其绿色安全、跨尺度、稳定性和良好的缓/控释性。因此，本项目将用共轭化-诱导组装-自交联耦合的微加工过程，从天然亚油酸(LA)制备有序微胶囊(OMC)。用化学/酶法使LA共轭化，获得兼具生物活性、表面活性和交联活性"三重"活性的关键单体共轭亚油酸(CLA)；通过分子组装技术获得CLA的胶束、囊泡、膜等有序分子组装体；最后引发其中双键适度自交联，获得具有成份绿色化、尺度多元化、构造有序化、形貌稳定化、载体功能化"五化"特征的OMC。拟采用DLS/SLS、cryo-TEM、ITC、AFM、荧光分子探针、金原位还原并沉积、毒性和缓释试验等方法表征产品和研究过程。本项目通过研究CLA的双键共轭化、分子有序组装、适度自交联等基本科学问题，深入探讨以生物质原料LA制备OMC的关键技术及耦合、微加工过程机制，为药品食品化妆品制造提供急需的具有"五化"特征的包埋缓释载体。

The desired characteristics of a releasing system should posses green and safety, multiscale, stable conformation and sustained/controlled release, which is difficult compatible in one embedding and sustained releasing system. In this research, ordered-microcapsules (OMC) will be microfabricated from linoleic acid (LA) through an integrated process of conjugation, induced assembly and self-crosslink. First, LA will be converted to conjugated linoleic acid (CLA) chemically or enzymatically with beneficial bioactivity, surface activity and crosslinking activity. Then, CLA will be induced assembling into aggregates such as micelles, vesicles or membranes. Finally, the moderate self-crosslink of the double bonds in CLA aggregates will microfabricate the green, safe, stable and sustained/controlled releasing OMC with multiscale and various conformations. The integrate process, microfabrication mechanism and the characteristics of OMC will be investigated using DLS/SLS, cryo-TEM, ITC, AFM, fluorescent molecular probe, in situ reduction gold-doping, toxicity and sustained release tests. By studying the fundamental scientific issues in the integration of conjugation, induced assembly and self-crosslink, some key techniques, the integrated technology and the microfabrication mechanism in the production of OMC from biomass LA will be explored intensively; and therefore providing the stable, embedded and sustained release system with multiscale and various conformations to the urgent needs in medicine, food and cosmetics fields.