负链RNA病毒基因组进入细胞经复制产生互补RNA才作为mRNA，是病毒分类的重要依据，能引起西瓜等作物发病。滇重楼病毒病报道较少，近年调查发现在云南有发生，对生产造成影响。NGS技术已成为发现鉴定病毒的新途径，对新发现的负链RNA病毒进行致病性鉴定是防控病害前提。在前期基础上，从滇重楼转录组数据中发现一负链RNA病毒序列，经RT-PCR证实存在该病毒，在RdRp核心区域有特征基序SDD和EFNS，与Bunyavirales成员RdRp氨基酸序列同源性仅达32%，分类地位待定。本研究通过设计背向背引物，扩增病毒全长序列并构建侵染性克隆及植物表达载体，用摩擦接种和农杆菌侵染接种寄主和指示植物，确定寄主范围并鉴定致病性。同时调查田间发生分布，确定病毒和田间症状之间的对应关系。首次在滇重楼上发现负链RNA病毒，明确其分类地位、寄主范围及致病性不仅为诊断防控奠定基础，也对研究其流行规律具有重要意义。

After the genome of -ssRNA virus getting into cells, the complementary RNA is reproduced and used as mRNA, which is an important basis for virus classification. the -ssRNA viruses could cause viral diseases on watermelon and other crops. There are few reports of the viral disease in Paris polyphylla var.yunnanensis, in recent years we found that viral diseases occured in Yunnan, which has an effect on production. NGS technology has become a new way to detect and identify viruses. Pathogenicity identification of newly discovered -ssRNA virus is a prerequisite for Disease control. On the basis of the preliminary data, the -ssRNA virus sequence was found from Paris polyphylla var.yunnanensis by NGS. The existence of the virus was confirmed by RT-PCR. In the core region of RdRp, characteristic sequences SDD and EFNS existed. It shared identities of 32% at amino acid level to the RdRp of all available Bunyaviales viruses. In this study, the full length genome of the virus would be amplified by designing back-to-back primers, and a cDNA infectious vector would be constructed and transformed into differential hosts. The hosts and indicator plants would be inoculated to study the host range of the virus and its pathogenicity,the field distribution also be investigated to identify correlation between the virus and symptoms. It is the first time to report -ssRNA virus on Paris polyphylla var.yunnanensis. It is important to study its classification, host range and pathogenicity, and it can supply the basis for further studying on control and epidemiology of viral disease.