蛋白质的O-连接的N-乙酰葡萄糖胺（O-GlcNAc）修饰能影响肿瘤细胞的黏附与恶性转移，有研究表明，调控细胞黏附涉及到细胞骨架蛋白中的重要成员Talin1，我们在前期研究中也发现O-GlcNAc修饰通过调控Talin1相关的黏着复合体形成可以促进宫颈癌细胞迁移。然而，宫颈癌中Talin1如何发挥其功能至今尚未有专家或学者进行深入研究。本项目对细胞骨架蛋白Talin1的O-GlcNAc修饰在宫颈癌黏附和转移中的作用进行了研究。在前期工作的基础上，本项目重点讨论：1.宫颈癌中Talin1的O-GlcNAc修饰与磷酸化修饰的相互影响。2.Talin1是否通过蛋白质的O-GlcNAc修饰发挥其调节宫颈癌细胞的黏着和转移功能？这些问题的阐明，将率先揭示出Talin1在宫颈癌细胞黏着与侵袭转移中的机制，为研究O-GlcNAc修饰在宫颈癌中的功能提供新的线索。

The O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins can affect the adhesion and metastasis in tumor cells. Studies have shown that the regulation of cell adhesion involves Talin1, which is an important member of the cytoskeleton proteins. In previous studies, we also found that the protein O-GlcNAcylation can promote cervical cancer cell migration by regulating the formation of Talin1-related adhesion complex. However, no experts or scholars conducted intensive research that how Talin1 performed the functions in cervical cancer. In this project, we will investigate the role of Talin1 O-GlcNAcylation in cervical cancer cell adhesion and metastasis. Based on previous work, we will focus on: 1. The interaction between O-GlcNAcylation and phosphorylation of Talin1 in cervical cancer. 2. Whether Talin1 regulates the cell adhesion and metastasis in cervical cancer through O-GlcNAc modification? We will be the first to clarify of the mechanism of Talin1 in cervical cancer cell adhesion, invasion and metastasis via these problems definition. We will also provide new clues for exploring the function of O-GlcNAc modification in cervical cancer.