自体脂肪组织是修复大体积软组织缺损的理想材料，但是传统的工程化的脂肪组织由于构建体积十分有限难以达到临床应用的需要。本课题组前期研究发现，通过改变力学微环境，可以诱导由干细胞参与的成熟脂肪组织的再生，构建大体积脂肪组织。研究表面脂肪组织内的细胞，如ASCs和血管内皮细胞都具有机械敏感性和机械反应性。因此我们推测，机械力通过改变细胞应力稳态使细胞骨架重排，促进细胞增殖，当细胞适应机械力改变，细胞内外张力重新达到平衡后，启动增殖细胞成脂分化。本研究拟围绕机械力启动脂肪组织再生及其调控机制展开，采用机械力细胞培养模型，蛋白生化，基因检测及荧光染色等技术，重点研究促进细胞增殖、成血管化和成脂分化的最适机械力参数，参与再生细胞来源，和大体积脂肪组织构建远位转移三个部分内容，阐明生物机械力与脂肪再生的密切相关性，通过非侵入性诱导脂肪组织再生，利用显微外科技术实现异位构建、游离移植的临床需要。

Autologous fat is the ideal material for repairing large volume soft tissue defect. However，the size of traditional engineered adipose tissue cannot meet the clinical requirement. Our group found that the change of mechanical microenvironment can induce regeneration of mature adipose tissue to reconstruct large volume adipose tissue using engineering chamber technique.Some studies suggested that cells in adipose tissue, such as ASCs and endothelial cells are mechanosensitive and mechanoresponsive. We provide the hypothesis that mechanical force promote cell proliferation via rearrangment of cytoskeleton and trigger adipogenesis when cells adapt to mechanical force. The following researches will focus on the mechanical parameter, the source of adipogenic cells, and reconstruction of large volume adipose tissue by mechanical force, and explore the relationship between biomechanical force and adipogenesis. This research is believed to be helpful for better understanding the mechanism of adipogenesis induced by mechanical force.