冠心病血瘀证脂质代谢谱及其模糊极大熵诊断模型的构建

本项目拟采用代谢组学研究策略，在我们已构建的代谢组学技术平台上，利用核磁共振（NMR）、气质联用（GC－MS）、液质联用（LC－MS）等研究手段，建立冠心病血瘀证脂质代谢指纹图谱，并对脂质代谢指纹图谱进行定性、定量分析，应用化学计量学方法进行模式识别，与冠心病痰浊证及正常人脂质代谢指纹图谱进行比较，建立判别冠心病血瘀证的模糊极大熵诊断模型，寻找冠心病血瘀证的相关分子标记物。并以方测证，用血府逐瘀汤干预后进一步验证"血瘀证"的可靠性及稳定性，从而在分子水平上阐明冠心病血瘀证的证候本质及血府逐瘀汤的治疗机理。有望为中医证候本质基础研究提供新的思路，构建一个科学评价中医证候的技术平台，为中医药现代化研究提供科学依据。

The project aims to use nuclear magnetic resonance (NMR), GC (GC-MS), liquid chromatography-mass spectrometry (LC-MS) and other research tools to establish lipid metabolism of blood stasis of coronary heart disease fingerprint. We investigate lipid metabolism fingerprint with qualitative and quantitative analysis. Firstly, we utlize chemometric methods to achieve the goal of pattern recognition. Then, in order to find related molecular markers, we establish fuzzy maximum entropy diagnostic model of blood stasis of coronary heart disease by comparing turbid phlegm of coronary heart disease with normal about lipid metabolism fingerprint. Lastly. Xuefuzhuyutang is used to intervention "blood stasis syndrome" to prove it reliability and steadiness, it will clarify the mechanism of coronary heart disease on molecular level and therapeutic mechanism of Xuefuzhuyutang. This will be useful for us to study TCM symptoms.