碳黑是一种应用广泛的工业产品，因其质量轻、粒径小，极易漂浮于空气中通过呼吸道进入人体而导致健康损害。IARC的评估报告认为碳黑可致遗传损害，但缺乏单纯碳黑暴露人群流行病学数据，导致碳黑致遗传损伤和肺癌的人群证据不足，并且损伤机制不清。本项目建立了单纯的职业碳黑暴露队列，同时建立吸入染毒的小鼠模型和细胞染毒模型，以课题组前期发现的纳米级碳黑诱导的损伤与FOXO信号有关的研究结果为切入点，采用基因克隆、ChIP、流式细胞分选等技术，将现场与实验室研究有机结合，探讨纳米级碳黑致DNA损伤、基因突变等遗传毒性，寻找纳米级碳黑在致人群呼吸系统疾病和遗传损伤终点出现之前的早期健康效应指标，阐明FOXO介导的信号途径在纳米级碳黑遗传毒性中的作用机制。

As an industrical product, carbon black floats in the air easily and induces damages to health. In the evaluation report of IARC, carbon black can induce genetic damages and there is inadequate evidence in humans for the carcinogenicity of carbon black because of the lacking of epidemiological evidences solely exposed to carbon black. The mechanism of damages induced by carbon black is not known very clearly. In the present project, the unique carbon black packers are chosen as the research objects. At meantime, the mouse and cell line will be used to establish the nanoscaled carbon black particles exposure models. In our previouse study, we found the mechanism of damages induced by nanoscaled carbon black particles was regulated by FOXO. Based on these results, the cell colony and flow cytometry sorting were used to evaluate the ability of cell transformation and gene mutation. The gene recombination、kinase activity analysis、ChIP、qRT-PCR, Western blot was used to test the genotoxicity and the mechanism through FOXO signal induced by carbon black. The present project will look for the sensitive biomarkers and early health effect indexes induced by nanoscaled black carbon particles before the advent of respiratory diseases and genetic damages end point by combination of field and laboratory research. The regulation of FOXO will be studied to explore the mechanism of genotoxicity induced by nanoscaled carbon black particles.