A high-density genetic map identifies a novel major QTL for boron efficiency in oilseed rape (Brassica napus L.).
A high-density genetic map identifies a novel major QTL for boron efficiency in oilseed rape (Brassica napus L.).
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高密度遗传图谱鉴定了油菜 (Brassica napus L.) 硼效率的新主要 QTL
DOI：
10.1371/journal.pone.0112089
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Xu F
中科院分区：
文献类型：
--
作者：
Zhang D;Hua Y;Wang X;Zhao H;Shi L;Xu F
低硼（B）严重限制了油菜的生长，对低B条件敏感的高B需求物质。在B人群中观察到对B缺乏反应的显著基因型变异。油菜品种揭示B中B效率的遗传基础。以甘蓝型油菜为材料，利用硼高效亲本青优10号与硼低效亲本Westar 10号杂交获得的双单倍体（DH）群体，分析了植株生长性状、B吸收性状和B效率系数（BEC）的数量性状位点（QTL）。利用芸苔属60 K Infinium微珠芯片、简单重复序列（SSR）和扩增片段长度多态性（AFLP）技术，构建了一张高密度遗传图谱。该连锁图总长度为2139.5cM，包含19个连锁群，相邻标记间的平均距离为1.6cM。通过对6个B效率性状的3个重复试验的水培评价，共检测到52个QTL，占表型变异的6.14-46.27%。在低B胁迫下，一个BEC主效QTL qBEC-A3a与其他生长和B吸收性状QTL共定位在A3上。使用置换系的子集，验证qBEC-A3a并将其缩小到CNU384和BnGMS436之间的区间。本研究的结果提供了一个新的主基因座位于A3的B效率在B。为甘蓝型油菜B效率的精细定位和分子标记辅助选择育种提供了依据。油菜。
Low boron (B) seriously limits the growth of oilseed rape (Brassica napus L.), a high B demand species that is sensitive to low B conditions. Significant genotypic variations in response to B deficiency have been observed among B. napus cultivars. To reveal the genetic basis for B efficiency in B. napus, quantitative trait loci (QTLs) for the plant growth traits, B uptake traits and the B efficiency coefficient (BEC) were analyzed using a doubled haploid (DH) population derived from a cross between a B-efficient parent, Qingyou 10, and a B-inefficient parent, Westar 10. A high-density genetic map was constructed based on single nucleotide polymorphisms (SNPs) assayed using Brassica 60 K Infinium BeadChip Array, simple sequence repeats (SSRs) and amplified fragment length polymorphisms (AFLPs). The linkage map covered a total length of 2139.5 cM, with 19 linkage groups (LGs) and an average distance of 1.6 cM between adjacent markers. Based on hydroponic evaluation of six B efficiency traits measured in three separate repeated trials, a total of 52 QTLs were identified, accounting for 6.14–46.27% of the phenotypic variation. A major QTL for BEC, qBEC-A3a, was co-located on A3 with other QTLs for plant growth and B uptake traits under low B stress. Using a subset of substitution lines, qBEC-A3a was validated and narrowed down to the interval between CNU384 and BnGMS436. The results of this study provide a novel major locus located on A3 for B efficiency in B. napus that will be suitable for fine mapping and marker-assisted selection breeding for B efficiency in B. napus.
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DOI：
10.1093/dnares/dsu002
发表时间：
2014-08
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Li F;Chen B;Xu K;Wu J;Song W;Bancroft I;Harper AL;Trick M;Liu S;Gao G;Wang N;Yan G;Qiao J;Li J;Li H;Xiao X;Zhang T;Wu X
通讯作者：
Wu X
影响因子：
7.4
作者：
Kobayashi, M;Matoh, T;Azuma, J
通讯作者：
Azuma, J
影响因子：
3.1
作者：
Li, Haitao;Chen, Xun;Liu, Kede
通讯作者：
Liu, Kede
影响因子：
7.2
作者：
Miwa, Kyoko;Takano, Junpei;Fujiwara, Toru
通讯作者：
Fujiwara, Toru
影响因子：
56.9
作者：
O'Neill, MA;Eberhard, S;Darvill, AG
通讯作者：
Darvill, AG