鼻咽癌（NPC）高转移严重影响NPC有效治疗，前期研究发现DNP诱导NPC转移伴AGR2高表达，分子模拟显示DNP与Agr2启动子结合，阻断Agr2基因，Cathepsin B、D表达下降，细胞转移降低。我们推断：DNP上调AGR2介导Cathepsin B、D表达，促进NPC转移。本项目拟进行: ①分析DNP诱导NPC细胞转移及AGR2、Cathepsin B、D表达；②分析沉默Agr2对DNP诱导Cathepsin B、D表达和细胞转移的影响及沉默Cathepsin b,d基因是否抑制DNP诱导细胞转移；③DNP对Agr2表达调控启动子分析；④动物实验分析Agr2、Cathepsin b或d 沉默DNP介导的细胞转移。⑤分析NPC患者DNP、AGR2与肿瘤转移的关系。阐明DNP通过 AGR2/Cathepsin B、D参与NPC转移的分子机制，为NPC转移分子机理研究提供科学理论依据

The metastasis of nasopharyngeal carcinoma (NPC) impact NPC therapy. Our previous works found that Dinitrosoperazine (DNP) induces NPC metastasis with a high expression of Anterior gradient-2 (AGR2)，molecule simulation showed that DNP interacts with the promoter of Agr2 gene, while Cathepsin B,D expression and cell metastasis significantly decrease while Agr2 blocked. We speculate that DNP may up-regulate AGR2, mediate Cathepsin B、D expression, and promote NPC metastasis. In this study, ① AGR2,Cathepsin B and D expressions are detected in NPC cells with DNP treatment and its metastasis is also measured. ② Cathepsin B,D expressions are detected in the Agr2 -blocked NPC cells and its metastasis is measured after DNP treatment. The cell metastasis is also detected when Cathepsin b or d gene blocked. ③ Agr2 gene promoter activity is analyzed followed DNP treatment. ④ When Agr2、Cathepsin b or d gene is blocked, DNP-mediated cell metastases are tested using nude mice. ⑤The relationships of DNP concentration, AGR2 level and NPC metastasis are analyzed in NPC patients. We will prove DNP-AGR2-Cathepsin B、D-metastasis signal pathway. This will provides a novel clue for NPC metastasis research.