肠道稳态与肠脑轴理论是近年药物作用机制研究的热点，调控肠内菌以改善病理状态是药物研发新方向。菊粉等天然果聚糖能正向干预肠内菌代谢而发挥调控血糖及免疫调节作用。限于结构复杂及获得性困难，制约了果聚糖肠内菌代谢调控等生物学作用研究的深入，利用化学合成手段获取结构确证的果寡糖是解决当前研究瓶颈的关键。果聚糖中主要的2,3-cis-呋喃果糖苷键构建目前仍是糖化学合成的难题。课题拟在前期发现的基于氢键介导分子内苷元传递策略高立体选择性构建2,3-cis-呋喃果糖苷键新方法的基础上，进一步优化、拓展新策略，构建骨架、位点、修饰等不同的结构多样果寡糖库及工具探针，评价其肠内菌代谢调控活性，获取有价值的构效关系及活性先导分子，初步阐明果寡糖调节肠内菌代谢影响神经功能的机制，为发展新型神经系统调节药物奠定基础。研究结果对丰富果寡糖合成化学、明晰果寡糖在肠内菌代谢中的调控机制、指导果寡糖药物开发具有重要意义。

Intestinal homeostasis and gut-brain axis theory are hot topics in recent years, and the regulation of intestinal bacteria as a means to improve the pathological state is a new direction of drug development. Natural fructan such as inulin can positively interfere with the metabolism of intestinal bacteria and play a role in regulating blood sugar and immune regulation. Due to the complexity of the structure and the difficulty of acquisition, the related biological functions on the metabolism and regulation of the intestinal bacteria have been hindered. The use of chemical synthesis to obtain structurally confirmed fructooligosaccharides is the key to solving the current research bottleneck. The construction of the 2,3-cis-fructofuranoside linkage is still a great challenge in carbohydrate chemistry. Based on our previous work using the hydrogen bond-mediated intramolecular aglycone transfer strategy to construct 2,3-cis-fructofuranoside bond, this project aims to further optimize and expand new strategies to construct different structural oligosaccharide libraries and tool probes, evaluate the metabolic regulation activities of intestinal bacteria and obtain valuable structure-activity relationship and active lead molecules. The mechanism that fructooligosaccharides regulate intestinal metabolism and affect neurological function will lay the foundation for the development of new neurological regulatory drugs. The above studies are of great significance for filling the blank of fructooligosaccharide synthesis chemistry, illuminating the regulation mechanism of fructooligosaccharides in intestinal bacteria metabolism, and guiding the medicinal use of fructooligosaccharides.