基于磁-力-电耦合的MNs-AgNWs/PDMS复合微薄膜生物传感器研究

表面应力生物传感器具有灵敏度高、检测特异性强等特点而成为研究热点，为了进一步突破AgNWs/PDMS复合微薄膜表面应力生物传感器的灵敏度，本项目拟引入磁性纳米材料（MNs），利用MNs-AgNWs/PDMS生物传感器实现待测物的分子级检测。项目针对复合微薄膜生物传感器的磁-力-电耦合及单分子识别传感机理等关键科学问题开展研究；解决高灵敏MNs-AgNWs/PDMS复合微薄膜生物传感器的设计与精准制备难题，突破纳米材料与柔性薄膜相结合的一致性加工难点；建立MNs-AgNWs/PDMS复合薄膜磁-力-电耦合的电阻式传感平台，实现检测灵敏高、操作简单、方便携带的复合薄膜生物传感器分子级检测方法；针对脑脊液中Tau蛋白进行检测，揭示MNs-AgNWs/PDMS复合微薄膜表面应力生物传感器高灵敏检测机制，为实现Tau蛋白的分子级检测提供支持。

Surface stress biosensor has become a research hotspot due to its high sensitivity and strong detection specificity. To improve the sensitivity of surface stress biosensor based on the AgNWs/PDMS composite membrane, this program will construct MNs-AgNWs/PDMS biosensor to achieve single molecular detection through introducing magnetic nanomaterials (MNs). This project will investigate the key scientific issues such as the magnetic mechanical electric coupling and single molecular detecting mechanisms of composite membrane biosensor, challenge for designing and preparing highly sensitive MNs-AgNWs/PDMS composite membrane biosensors accurately, solve the difficult processing of the nanomaterials combining with flexible membrane. A magnetic mechanical electric coupling resistance sensing platform with MNs-AgNWs/PDMS composite membrane will be built to realize high sensitive, simple operation and convenient carrying for molecular detection. Furthermore, based on Tau protein detection, this project will reveal the highly sensitive detection mechanism of MNs-AgNWs/PDMS composite membrane surface stress biosensor, which will provide theoretical support for single molecular detection.