性信息素合成激活肽（Pheromone Biosynthesis Activating Neuropeptide，PBAN）是调控蛾类性信息素生物合成的神经激素。其作用方式是通过调控脂肪酸前体和脂肪酸的合成来实现的。前期研究证实，PBAN通过钙调磷酸酶激活乙酰辅酶A羧化酶从而调控脂肪酸的合成。然而对PBAN如何调控脂肪酸前体合成的研究还处于空白。本申请项目前期工作中首次发现4个受PBAN调控的糖转运蛋白及其磷酸化变化，然而详细的调控机制尚不明了。本项目以农业害虫棉铃虫为材料，拟采用RNAi、荧光定位、免疫沉淀、GC/MS等生理生化和分子生物学方法，对性信息素腺体糖转运蛋白的分子特性、糖转运活性，对性信息素合成的影响及PBAN对其调控的分子机制进行深入研究。该研究不仅丰富PBAN对性信息素合成调控的分子机制、理解昆虫补充营养对昆虫发育的科学意义，并为筛选害虫防治新靶标提供科学依据。

Pheromone Biosynthesis Activating Neuropeptide (PBAN) is a neuropeptide which regulates the sex pheromone biosynthesis in moths. PBAN role is via G-coupled protein receptor signal transduction pathway to mediate the biosynthesis of fatty acid precursor and fatty acid, therefore mediating sex pheromone biosynthesis. Our previous study confirmed that PBAN mediated sex pheromone biosynthesis by calcineurin to activate acetyl-CoA carboxylase, therefore to regulate fatty acid biosynthesis. However, the study concerning PBAN regulation of the fatty acid precursor biosynthesis still remain elusive. Our previous study also demonstrated that 4 sugar transporters were regulated by PBAN via phosphorylation/dephosphorylation, however the detailed mechanisms of PBAN regulation of sugar transporters are unknown. In this study, the molecular characterization, sugar transported activity of sugar transporter, the effect of sugar transporter on sex pheromone biosynthesis and PBAN-regulated mechanism of sugar transporter will be studied using RNAi、fluorescence localization, immunoprecipitation, GC/MS methods and so on by using Helicoverpa armigera as model. This study not only illuminate the molecular mechanism of PBAN-regulated sex pheromone biosynthesis and understand the scientific significance of diet supplement on insect development, but also provide scientific base for screening new target of pest control.