MAPK/ERK信号通路参与PBAN调控家蚕性信息素生物合成分子机制的解析

性信息素合成激活肽（Pheromone Biosynthesis Activating Neuropeptide，PBAN）调控性信息素生物合成。在以家蚕（Bombyx mori）等为模式的研究中发现，PBAN通过调控脂滴的脂解从而调控了性信息素的生物合成。本研究前期实验发现PBAN通过招募MAPK/ERK信号，从而调控了脂滴的脂解和性信息素的生物合成，但详细的分子机制尚不清楚。本项目以B. mori为模式，拟采用磷酸化蛋白质组学、PRM分析、双荧光素分析和RNAi分析等方法，研究PBAN如何激活MAPK/ERK信号途径，MAPK/ERK信号途径在脂滴脂解和性信息素生物合成中的功能及MAPK/ERK信号途径下游靶标的鉴定及功能分析等。该研究可揭示MAPK/ERK信号调控脂滴脂解及性信息素合成与释放的分子机制，为害虫防治新靶标的挖掘提供科学依据。

Pheromone biosynthesis activating neuropeptide (PBAN) regulate sex pheromone biosynthesis. In Bombyx mori, PBAN regulates sex pheromone biosynthesis via mediating the lipolysis of lipid droplets in pheromones gland. Our previous studies confirm that PBAN regulats the downstream signal molecules by recruiting MAPK/ERK signals, therefore to regulate the lipolysis of lipid droplets and subsequent sex pheromones biosynthesis. However the detailed molecular mechanism concerning MAPK/ERK signal transduction in the lipolysis of lipid droplets and subsequent sex pheromones biosynthesis still remains unknown. In present study, B.mori is used as model to study how PBAN activates MAPK/ERK signaling pathway, the function of MAPK/ERK signaling pathway in lipid drop lipolysis and sex pheromone biosynthesis, and the identification and functional analysis of downstream targets of MAPK/ERK signaling pathway using phosphoproteomics, PRM, bisluciferin analysis and RNAi technologies. This study may reveal the molecular mechanism of lipid droplet lipolysis and sex pheromone biosynthesis regulated by MAPK/ERK signal. It also provides scientific foundation for the excavating new targets for pest control.