Supplemental Sugar Is Required for Sex Pheromone Biosynthesis in Mythimna separata.
Supplemental Sugar Is Required for Sex Pheromone Biosynthesis in Mythimna separata.
复制标题
粘虫的性信息素生物合成需要补充糖。
DOI：
10.3389/fphys.2020.605145
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发表时间：
2020
影响因子：
4
通讯作者：
Yin X
中科院分区：
文献类型：
--
作者：
Zhang Y;Zhang Y;Yao S;Wang G;Wei J;Du M;An S;Yin X
补充营养的成年蛾最大限度地提高蛾的健身和有助于许多植物的授粉。以往的研究表明，糖食通过增加交配蛾血淋巴中海藻糖的浓度来促进性信息素的合成。以粘虫Mythimna separata成虫为模型，研究了糖对粘虫性信息素合成的影响。结果表明，糖食显著提高了雌蛾性信息素腺体中海藻糖、维生素C和酰基辅酶A的含量，从而提高了雌蛾性信息素的滴度、吸引雄蛾的能力和交配成功率。一致的是，喂糖的雌性比喂水的雌性产更多的卵。此外，饥饿的雌性的再喂养也引起了海藻糖的浓度显着增加，葡糖酸，和酰基辅酶A的PGs，从而促进性信息素的生产显着增加。最重要的是，RNAi介导的海藻糖酶敲低（导致PG饥饿）导致海藻糖含量增加，PG中的海藻酸和酰基辅酶A浓度降低，这反过来又导致性信息素滴度降低，雌性吸引雄性的能力和成功的交配效率降低。结果表明，糖食促进了海藻糖的利用，并通过提高海藻酸和酰基辅酶A的浓度，促进性信息素前体的合成，从而促进了性信息素的合成和交配成功。
Supplemental nutrients of adult moths maximize moth fitness and contribute to the pollination of many plants. Previous reports have revealed that sugar feeding promotes to sex pheromone biosynthesis by increasing the haemolymph trehalose concentration in mating moths. Here, Mythimna separata adults were employed as a model to investigate the effect of sugar feeding on sex pheromone biosynthesis. Results showed that in virgin females, sugar feeding markedly increased the concentrations of trehalose, pyruvic acid, and acyl-CoA in pheromone glands (PGs), which in turn led to an increase in sex pheromone titer, female ability to attract males and successfully mating frequency in sugar-fed females. Consistently, sugar-fed females laid more eggs than water-fed females. Furthermore, the refeeding of starved females also caused significantly increase in the concentrations of trehalose, pyruvic acid, and acyl-CoA in PGs, thus facilitating a significant increase in sex pheromone production. Most importantly, RNAi-mediated knockdown of trehalase (leading to PG starvation) resulted in an increase in trehalose content, and decrease in the concentrations of pyruvic acid, and acyl-CoA in PGs, which in turn led to a decrease of sex pheromone titer, female ability to attract males and successful mating efficacy. Altogether, results revealed a mechanism by which sugar feeding contributed to trehalose utilization in PGs, promoted to significantly increased sex pheromone precursor by increasing the concentrations of pyruvic acid and acyl-CoA, and facilitated to sex pheromone biosynthesis and successful mating.
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影响因子：
3.7
作者：
Du M;Yin X;Zhang S;Zhu B;Song Q;An S
通讯作者：
An S
影响因子：
3.8
作者：
Fan, YL;Rafaeli, A;Applebaum, SW
通讯作者：
Applebaum, SW
影响因子：
3
作者：
Lee, DW;Boo, KS
通讯作者：
Boo, KS
酪氨酸羟化酶和多巴脱羧酶的过度表达与甜菜夜蛾蛹黑色化相关。
影响因子：
4.6
作者：
Liu S;Wang M;Li X
通讯作者：
Li X
DOI：
10.1073/pnas.1632485100
发表时间：
2003-08-19
影响因子：
11.1
作者：
Choi, MY;Fuerst, EJ;Jurenka, R
通讯作者：
Jurenka, R