鸡性别控制是全球家禽业最引人关注的重大问题之一，是关系到改变家禽生产技术创新能否实现的关键。但目前调控性别分化发生的关键基因网络机理仍不清楚，给鸡智能集约化生产造成极大束缚。本课题前期发现了调控性别分化的新候选基因，且与已知基因及芳香化酶信号通路构成了级联和拮抗调控关系。根据这些发现本课题提出了性别分化相关基因之间及其与性激素信号通路应答网络调控机制假说。拟用获得性和缺失性等方法明确候选基因与已知基因及其与信号通路之间的表达规律和关系；利用蛋白质互作技术研究候选基因与已知基因及与信号通路节点基因互作机制及其对性别分化的调控机制；采用RIP和蛋白质互作等方法探明候选基因在转录和转录后及其表达产物的泛素化修饰对性别分化的作用机制；结合生物信息学方法筛选并验证Dmrt1基因特异抑制剂对性别分化的干预效果。为进一步阐明性别分化的发生机制提供新理论证据，为家禽性别控制技术创新提供可靠的支持依据。

Chicken sex control is one of the major concern in the global poultry industry, and the key step to achieve the technological innovations to improve poultry production. However, it is still unclear the regulatory network mechanism of key genes involve in sex differentiation, which results great constraints on chicken intelligent intensive production. The preliminary research of this project found many candidate genes regulate sex differentiation. These genes also form a cascade and antagonistic relationship with known genes and aromatase signal pathways. This project will clarify the refined mechanism between genes, genes and sex hormone signal pathways, via methods of acquisition and deletion, etc.; Interactive mechanism between candidate genes, known genes and key genes of signal pathways will be elucidated by protein interactive technology; RNA-protein interaction technology will be used to explore pre-transcriptional and post-transcriptional ubiquitin modification of candidate gene during sex differentiation. Finally, we will combine the bioinformatics to select and verify the specific inhibitors of gene DMRT1 to interfere the sex differentiation. All these studies will provide a new theoretical evidence for further mechanism clarifying of sex differentiation, and pave a fundamental way to improve the sex identification technology.