长链非编码RNA MALAT1调控口腔鳞状细胞癌炎症微环境机制的研究

慢性炎症是肿瘤的基本特征之一，在口腔鳞状细胞癌发生发展中起着重要作用。长链非编码RNAs是近年来发现的一类转录本超过200 nt的ncRNAs。研究表明lncRNA MALAT1在人类多种肿瘤中可提高肿瘤细胞增殖、转移和侵袭能力；而NF-κB信号通路在口腔肿瘤炎症环境的改变中具有重要作用。本课题组前期研究显示MALAT1在口腔鳞状细胞癌中高表达，沉默MALAT1后，基因芯片显示各类肿瘤生物学行为相关因子如炎症因子的改变。因此我们猜测MALAT1在口腔鳞状细胞癌的炎症微环境起着重要的作用。本研究旨在从细胞、分子及动物水平系统地探讨口腔鳞状细胞癌炎症微环境中MALAT1及NF-κB信号通路的作用及对T细胞、巨噬细胞及免疫相关因子的作用机制。本研究预期结果不仅能为口腔鳞状细胞癌与炎症建立一个新的联系，也能为口腔鳞状细胞癌的预防和治疗提供理论依据及一个新的治疗靶点。

Chronic inflammation is one of the basic characteristics of the tumor, it plays an important role in development of oral squamous cell carcinoma. Long non-coding RNAs (lncRNAs) which is discovered in recent years is longer than 200nt. Rencent studies have shown that lncRNA MALAT1 can improve the ability of cell proliferation, metastasis and invasion in lots of human tumors. Moreover, the NF-kappa B signaling pathway plays an important role in the inflammation microenvironment. In our research, we find the high expression of MALAT1 in oral squamous cell carcinoma, and when we silence this lncRNA, a lot of tumor biology behavior related factors including inflammatory factors are found to have changed. So we guess MALAT1 may play an important role in development of oral squamous cell carcinoma in the inflammatory microenvironment. Therefore this study is to explore the relationship between MALAT1 and inflammatory microenvironment in oral squamous cell carcinoma among the cell , molecular and animal level, and analyze its role in T cells, macrophages and immune related factors. Clarify the role of MALAT1 and NF-kappa B signaling pathway in the oral squamous cell carcinoma. This study can not only prove a new reletionship between oral squamous cell carcinoma and immune, but also provid a potential target for oral squamous cell carcinoma therapy.