金属有机骨架纳米模拟酶的功能化用于构建高选择性生物传感器

生理活性物质在生物体系内许多生理和病理过程中起着非常重要的作用，对这些物质建立快速简便的分析方法有助于深入认识生命过程的本质，因此具有非常重要的意义。本项目拟通过合理设计和合成，选取具有模拟天然酶催化活性的金属有机骨架作为生物传感平台，充分利用其结构特点，通过两种方式对其进行功能化：一是在合成金属有机骨架前对配体进行有针对性的修饰，使其具有特异性识别位点；另一是通过后合成修饰方法，将与靶标特异性结合的识别单元接枝到金属有机骨架上，或与其他纳米材料复合。由此构筑的高选择性生物分子识别界面可建立针对多种生理活性物质的生物传感器，并进一步实现对实际生物体系内的生理活性物质的细胞成像和实时监测等。本项目的实施不仅有望解决现有此类生物传感器存在的选择性差的突出问题，而且也将拓展金属有机骨架作为纳米模拟酶在生物研究领域中的应用。

Biolactive substances play a very important part in many physiological and pathological processes in living systems. It is thus of great significance to develop simple and fast analytical methods for them to deeply understand the essence of relevant biological process. In this project, we plan to select metal-organic frameworks (MOFs) with natural enzyme-like catalytic activities as biosensing platform and functionalize them with two manners by taking advantage of structural characteristics: one is target-directed modification of ligands before the preparation of MOFs to endow them with specific binding sites; the other is grafting recognition elements toward the targets onto MOFs or integrating them with other nanomaterials. The so-formed highly selective biointerfaces could be used for the construction of biosensors for bioactive substances, and further enable their cell imaging and real-time monitoring in living systems. This strategy will not only provide a promising tool to address the issue of poor selectivity in existing biosensors based on MOF nanozymes, but also broaden the biological applications of MOFs with enzyme-like catalytic activities.