以配位聚合物为比例型荧光探针的活体分析研究

生理活性物质参与一系列脑神经相关的生理和病理过程，在其中扮演了极为重要的角色。因此为了揭示脑神经过程的化学本质，建立和发展高选择性、高灵敏度的可用于实时检测脑内生理活性物质的简便快捷的分析方法具有非常重要的意义。本项目拟通过合理设计和合成，首先制备具有荧光性质的配位聚合物，并利用其对客体分子（如荧光生色团）的包裹特性，得到具有双发射性能的纳米复合材料，再通过待测物与上述两者之一的特异性结合，发生相应的荧光变化，从而构建基于配位聚合物的比例型荧光传感器，与活体微透析取样技术结合，即可实现活体分析中生理活性物质的高选择性、高灵敏和抗环境干扰的实时检测。本项目的实施不仅为发展简便快捷的活体分析方法提供了新的思路和方法，也将进一步开拓功能配位聚合物在实际生物研究领域中的应用前景。

Biological active species play very critical roles in various processes of physiology and pathology in brain, so it is of great importance to develop simple and fast analytical methods for their real-time monitoring with high sensitivity and good selectivity to understand the essence of related biological process. In this project, we plan to develop new sensing platforms using coordination polymer-based ratiometric fluorescent probes for determination of various biological active species in vivo with high sensitivity and specificity. Luminescent coordination polymers are synthesized to adaptively encapsulate guest molecules (i.e., fluorophores), resulting in dual-emission nanohybrids. For such fluorescent probe, specific recognition towards targets induces ratiometric fluorescence changes. Because of built-in correction for environmental effects, by combining with microdialysate technology, our proposed sensing platforms accomplish in vivo and real-time monitoring of biological active species in brain with high sensitivity and good selectivity. This strategy will not only open a new way to developing an effective methods for in vivo analysis in a very simple fashion, but also broaden the biological applications of functional coordination polymers.