利用GFP和RFP载体转化、抗体免疫荧光标记等方法研究AtSYP131等SNARE蛋白在花粉管内的分布和相关；利用T-DNA插入突变、过表达等方法，分析AtSYP131等Qa-SNARE蛋白在花粉管顶端生长方面的作用；利用酵母双杂交、pull-down、BiFC等方法，分析花粉管中SNARE蛋白AtSYP131、AtSNAP30及AtVAMP725间相互关系。在本项目的研究基础上，确认AtSYP131等SNARE蛋白在花粉管顶端囊泡分泌中的相关与作用。以期更深入理解特定SNARE蛋白复合体在花粉管顶端生长中的构成与作用。

By fluorecent labeling methods, coupling confocal microscopy observation, the distribution and relation of AtSYP131, AtSNAP30 and AtVAMP725 will be observed in pollen tubes. By T-DNA and overexpress methods, we wish to change express level of AtSYP131 gene in Arabidopsis, and analyse the function of AtSYP131 for exocytosis and tip-growth in pollen tube. By Yeast two-hybrid, GST pull-down and BiFC methods, we wish to analyse the relation between AtSYP131, AtSNAP30 and AtVAMP725. After this project finish, we wish to determine the structure and function of SNARE complex in pollen tube tip-growth. These results will help us to understand farther the mechanism of pollen tube tip-growth.