声波作为重要的环境因子，在海洋中无处不在，衰减极少，但其对水生无脊椎动物的影响则一直受到忽略。明确水生无脊椎动物的听力范围以及其对声波的行为和生理响应方式，对促进生物生长，提高动物福利有着重要意义。本研究拟以三疣梭子蟹为研究对象，以听性脑干反应测定其听力阈值和听频范围，并利用同步辐射等方法查明其听觉器官(平衡囊)结构。在此基础上，设置不同可闻声波处理组(莫扎特G大调小夜曲、罗茨鼓风机噪声)，一方面通过计算机视觉、社会网络分析等技术从运动摄食、相残行为等角度量化研究不同可闻声波下梭子蟹的行为差异；另一方面通过能量代谢、放射免疫和荧光定量等方法，探索不同可闻声波对梭子蟹能量收支、酶活力、神经递质水平和相关基因表达的影响，查明梭子蟹对特定可闻声波的行为和生理响应规律。本研究不但能够丰富对于水生无脊椎动物听觉能力的基本认知，还可从声学角度，为保护海洋蟹类资源，提高动物福利水平提供新思路。

As an important environmental factor, sound waves in the ocean are all around and with low attenuation, while their impact on invertebrate aquatic animal is poorly understood. Studying the scope of hearing ability of aquatic organisms and the effect of sound waves on their physiological status and behavior, would help to improve the welfare of the animal. In this study we intend to use auditory brainstem response to measure the hearing threshold and audible frequency range of the swimming crab portunus trituberculatus, then explicit the structure of statocyst by electron microscope and synchrotron radiation. Based on these results, the physiological and behavioral effect of audible sound wave on the animal were explored, i.e. energy budget, digestive enzyme, neurotransmitter and associated gene expression. This work firstly studies the hearing ability of crab, providing a way to protect marine crab and optimize the welfare of the animals.