在担子菌和子囊菌两类跨门物种之间，在子实体发育过程中存在一个形态发育过程非常类似的阶段-从营养菌丝分化为子实体雏形（原基或原子囊果）的阶段，对于这一共性形态发育过程中是否存在共性的分子调控机制，尚未有人解答。申请人课题组发现了担子菌、子囊菌中蛋白序列十分保守的、含有WD40重复结构域的蛋白CPC-2，其编码基因在金针菇、粗糙脉孢菌中都正向调控子实体的早期发育，而且金针菇中CPC-2的编码基因Fvcpc2转入粗糙脉孢菌Δcpc-2能使该突变体恢复产生原子囊果的能力，说明在担子菌、子囊菌之间存在调控子实体发育的共性调控元件。本项目拟研究CPC-2通过调控哪些基因的表达产生对原子囊果发育的调控作用、CPC-2通过与哪些蛋白形成复合体共同调控原子囊果的发育、CPC-2对子实体发育的调控模式在其他真菌中是否保守。项目的实施，有望找到信息素信号系统之外第二条对真菌有性发育具有普遍调控作用的信号途径。

Between two phyla of fungi, Ascomycota and Basidiomycota, a morphological development process, in which vegetative hyphae develop into protoperithecium or primordium, is very similar. It is unknown if there is a common element(s) to regulate this similar morphological development. We found a WD40-domain containing protein CPC-2 and its homologs from Ascomycota and Basidiomycota have very conserved amino acid sequences and demonstrated CPC-2 is essential for the initiation of fruiting body formation in both Neurospora crassa and Flammulina velutipes. Transformation of F. velutipes Fvcpc2 gene into N. crassa cpc-2 deletion mutant can restore the wild-type protoperithecium forming ability to the mutant, indicating a common element regulating the development of protoperithecium or primordium exists. In the proposed project, we will answer the following questions: 1) Which genes are regulated by CPC-2 and how do these genes contribute to fruiting body development? 2) Which proteins do CPC-2 interact with and how do them contribute to fruiting body development? 3) Are the regulatory mechanisms by CPC-2 conserved among fungi between different phyla? It is possible to find the second common regulatory pathway for fungal sexual development after the proposed plan is fulfilled.