由交链孢菌（Alternaria alternata）代谢产生的细交链格孢菌酮酸（TeA），是在农产品中检出率最高且毒性最大的交链孢毒素，同时也是重要的致病因子。已有研究表明真菌毒素的合成与菌体活性氧（ROS）代谢密切相关，而抗氧化剂可在一定程度上清除ROS。申请人前期发现，抗氧化剂厚朴酚对TeA的累积具有极显著的抑制作用。在此基础上，本项目通过厚朴酚及DPI（NOX专一阻断剂）处理，利用显微镜观察、生化测定、实时定量PCR及iTRAQ等技术，研究厚朴酚对TeA合成过程中关键差异蛋白的调控，并从转录水平对功能基因进行验证，结合厚朴酚对菌体质膜完整性和H2O2、AaAP1及NOX等相关ROS代谢体系的调控，进而揭示厚朴酚等抗氧化剂对真毒产毒的调控机制，以期为开展果蔬贮藏加工过程中利用抗氧化剂防控交链孢毒素提供科学依据。

Tenuazonic acid (TeA) produced by Alternaria alternata is one of Alternaria toxins with the highest and most toxic detection in agricultural products, but also an important virulence factor. Meanwhile, previous reports had shown that the reactive oxygen species (ROS) plays a pivotal role in the mycotoxins biosynthesis, and antioxidants can scavenge ROS in a certain extent. The high inhibition effects of magnolol on TeA accumulation was observed by our previous study. Therefore, in this proposal, by magnolol and diphenyl iodonium (DPI) treatment, microscopy observation, biochemistry analysis, RT-PCR detection and isobaric tags for relative and absolute quantitation (iTRAQ)-LC-MS/MS are employed to study the effect of magnolol on differential protein in TeA biosynthesis, and the functional genes were verified at the transcriptional level. Combined with the effect of magnolol on cell membrane integrity and ROS metabolism, the regulation mechanism of magnolol to TeA accumulation was revealed. The expected results would provide scientific reference for using antioxidants to reduce and remove the Alternaria toxins on agro-products during storage and processing.