维管形成层周期性活动是林木次生生长和木材形成的关键生物学过程，受到内源激素信号和外界环境因子的调控。形成层细胞如何感知并对相应信号做出正确的反应，从而调节形成层细胞活性状态是林木基础研究和林业生产广受关注的重要科学问题。我们前期研究揭示了质膜蛋白Remorin通过与受体激酶形成复合体调控植物信号转导的机制。在杨树中，我们发现一个Remorin基因参与了形成层活性调控。本研究将利用蛋白质组学方法，鉴定与Remorin形成复合体的蛋白；以基因编辑、生化和细胞生物学等技术手段，解析该Remorin及其复合体在形成层活动中的功能。通过对Remorin复合体的研究，阐释该杨树Remorin基因在调节维管形成层周期性活动的信号转导机理，为速生林木培育提供新基因资源和理论指导。

Vascular cambium shows a periodic activity for secondary growth and wood formation. Such periodic activity is regulated by plant hormone signals and environmental factors. How cambium cells perceive and response correctly to these signals, thereby to regulate cambium activity is a key question in understanding of tree growth and improvement. In previous studies, we demonstrated that remorin protein interacts with receptor-like kinase in regulation of signaling transduction in plants. In Populus, we identified a remorin gene which is involved in regulation of vascular cambium activity. In this project, we will employ proteomic approach to identify the proteins that interacts with this remorin. Then through analysis of CRISPR/Cas9 genome editing, biochemical and cellular technology, the remorin and its complex will be dissected for the function in related to vascular cambium activity. We expect the study will reveal the signaling process mediated by the remorin in regulation of vascular cambium activity in Populus. The new knowledge generated from this study would aid in innovation of technology for tree improvement.