基于质谱的复杂生物体系中关键化学物质分析新方法研究

复杂生物体系如体液等中存在着含量极少但功能非常重要的关键化学物质，如神经递质，植物激素和代谢物等。这些化学物质的分析对探讨生命现象的本质和疾病的预警等意义重大。由于复杂体系中高含量组分的干扰，使得其分析和检测异常困难。本申请旨在以质谱为主要研究手段，在复杂的生物体系中借助化学衍生、新型质谱基质开发、特异性识别等研究手段，发展针对神经递质、代谢产物等生命活动中重要的化学物质的灵敏、快速、实时的分析方法。为探讨上述化学物质的生理功能、相关疾病的预警、药物的治疗和临床基础研究等奠定基础。

Different kind of key chemical substrates with low amount but significant functions exist in the complicated bio-system, for example, body fluid. Because of the interference of other components in the complicate fluid, it is extremely difficult for the detection of those key chemical substrates. Relying on the chemical derivatization, novel SALDI matrix, special interaction between the analytes and the matrix, the sensitive, rapid, in situ analytical methods will be developed on the base of the Mass Spectrometric approach, which will provide fundamental data for the elucidation of the physiological function, diagnosis of diseases, medicine treatment and clinical research.