长链非编码RNA(lncRNA)是一类转录本长度超过200nt的RNA分子，它们并不编码蛋白，而是以RNA的形式在多种层面上调控基因的表达。关于lncRNA在细胞衰老过程中的作用目前仍不清楚。本课题以人正常二倍体成纤维细胞2BS为研究对象，探讨了lncRNA对细胞衰老的调节作用。在前期研究工作中，我们利用基因芯片筛选了2BS细胞衰老过程中差异表达的lncRNA， 并证实其中一个表达显著上调的lncRNA(登录号：BC012900. 暂时命名为lncRNAU5)与细胞衰老密切相关。接下来的研究我们将集中在两方面：（1）证实lncRNAU5作用的靶蛋白，明确lncRNAU5调节细胞衰老的分子机制；（2）证实细胞衰老过程中调节lncRNAU5表达的转录因子，明确在细胞衰老调控网络中以lncRNAU5为"节点" 的信号通路。以上研究将为细胞衰老及衰老相关性疾病的发生机制研究提供新的线索。

Long non-coding RNA (lnRNA) are a class of RNA with longer than 200nt, they do not encode protein, but regulate gene expression at a variety of levels. whether lnRNA regulates cellular senescence remains unclear. In this study, we investigate the effect of lncRNA on cells senescence with normal human diploid fibroblasts 2BS. In preliminary studies, we used gene chips to screen lncRNAs with differentially expression during senescent process, and confirmed that a lncRNA with significantly upregulated expression (accession number:. BC012900 temporarily named lncRNAU5) is closely related to cells senescence. Next, we will focus studies on two aspects: (1) Identifying lncRNAU5 target proteins to reveal molecular mechanisms underlying lncRNAU5 regulation of cells senescence; (2) Identifying transcription factors regulating lncRNAU5 expression in senescent cells to reveal signaling transduction pathway with lncRNAU5 as "nodes" in senescent cells。The above studies will provide new clues to investigate the molecular mechanisms of cellular senescence and senescence-related diseases.