白叶枯病是水稻的重要细菌性病害。在前期工作中我们发现来自于同一个MtN3基因家族的xa13和xa25基因均为抗病主效基因，而它们对应的显性Xa13、Xa25和该家族Os11N3基因分别受白叶枯病菌不同效应蛋白调控，造成水稻感病，但是这同一基因家族的三个成员之间关系尚不明确。本项目拟研究这三个基因在水稻-白叶枯病菌互作中彼此之间的生物学关系，主要内容包括：分析这三个基因转运糖类与白叶枯病菌侵害水稻之间的分子机制，重点分析它们转运糖类种类特异性和在特定细胞类型中转运方向性；确定协助白叶枯病菌效应蛋白特异调控这三个基因的辅助因子，解析这三个基因之间应对这些辅助因子甄别而进行博弈的分子机制；探索利用这些辅助因子在作物抗病遗传改良中途径；最终明确这三个基因在水稻-白叶枯病菌互作中彼此之间的分子协同互作或竞争关系。本研究不仅能够丰富对寄主-病菌过程互作复杂机理的认识，而且也为作物抗病育种提供新的思路。

Rice bacterial leaf blight was a serious disease caused by Xanthomonas oryzae pv.oryzae (Xoo). In the previous research we found the xa13 and xa25 belonging to MtN3 gene family were recessive disease resistance gene, whereas rice that containing the corresponding dominant allele Xa13, Xa25 and Os11N3 which were regulated by effectors of Xoo respectively, was susceptibility to Xoo. The relationship between the three genes was not clear until now. In this project we focus on the molecular relationship of the three genes belonging to the MtN3 gene family during rice-Xoo interaction, it including: analyse the mechanism of the three genes functioning in sugar transporting during Xoo infracting rice, mainly in transporting sugar varieties preferences and transporting cell types preferences; identify the helper proteins for Xoo effectors targeting the rice genes, specificity, and analyse the mechanism that how helper protein screening three genes after Xoo infecting; explore the new method to use the helper proteins in rice genetics improvement; identify the collaborative or competition mechanism among the three genes during rice-Xoo interaction. This research would broaden the molecular mechanism of host-microbe interaction, and also may provide new tactics for disease resistance breeding.