组蛋白H2B单泛素化参与VD-toxins胁迫应答及调控细胞微管骨架动态的作用机制

本项目利用拟南芥野生型和组蛋白H2B单泛素化基因缺失的系列突变体，以拟南芥幼苗与VD-toxins互作为研究体系，研究组蛋白H2B单泛素化参与VD-toxins胁迫应答及调控细胞微管骨架动态的作用机制。通过研究VD-toxins处理对野生型和突变体细胞微管骨架的时空动态变化、酪氨酸磷酸化信号通路和微管结合蛋白MAP65、MAP18活性及磷酸化水平的影响，从细胞、分子水平上阐述组蛋白H2B单泛素化调控微管骨架动态的作用机制，阐述组蛋白H2B单泛素化参与调控植物抗VD-toxins防卫反应中的功能，进而阐明表观遗传调控植物抗黄萎病作用机制的细胞学基础。本研究对阐明植物抗黄萎病的抗性机理具有重要意义，为全面揭示植物抗棉花黄萎病的表观遗传调控机制提供新内容，为利用基因工程手段控制棉花黄萎病开阔新领域。

In this research project, we will use wild-type and hub, ubc mutants that lack histone H2B monoubiquitination (H2B ub) of Arabidopsis to explore the molecular mechanisms of H2B ub is involved in regulating the dynamic microtubule cytoskeleton during the defence responses to VD-toxins. We will investigate that the role of H2B ub in the regulation of dynamic microtubules cytoskeleton, and the relation linking H2B ub with tyrosine phosphorylation/dephosphorylation processes mediated by protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs), and the activation of microtubule-associated proteins (MAPs) that control the dynamic properties and organization of microtubule may be regulated through tyrosine phosphorylation and dephosphorylation switches, and the changes in defense-related gene expression after treatment with VD-toxins. The results will provide evidence that H2B ub is involved in the regulation of microtubule assembly and disassembly and microtubule functions. The results will suggest a functional relationship between H2B ub and dynamic microtubule cytoskeleton and it will be helpful to reveal the molecular mechanisms of H2B ub controlling the defense response against VD-toxins. The results will provide novel insight into the mechanisms of epigenetic regulation in plant defence responses to Verticillium dahliae.