组蛋白H2B单泛素化调控微管骨架在信号网络核心的时空信号功能及响应盐胁迫的作用机制

本项目利用拟南芥野生型和组蛋白H2B单泛素化基因缺失的系列突变体，研究组蛋白H2B单泛素化调控拟南芥响应盐胁迫应答的作用机制。通过研究组蛋白 H2B单泛素化响应盐胁迫过程中微管骨架的时空动态、酪氨酸磷酸化调控微管骨架动态的作用机制、筛选HUB、UBC参与调控的靶基因和作用途径，揭示微管骨架解码盐胁迫的时空特性；微管骨架在信号网络系统中的核心功能；酪氨酸磷酸化的信号转导功能；酪氨酸磷酸化信号通路调控微管骨架时空动态的作用靶点及信号途径。从细胞、分子水平上阐述组蛋白H2B单泛素化调控微管骨架在信号网络核心的时空信号功能及响应盐胁迫的作用机制，进而阐明表观遗传调控植物响应盐胁迫应答的全新功能。本研究可进一步开阔植物耐盐信号转导机制研究的新领域，从而推动植物抗盐分子机理研究的深入。

In this research project, we will use wild-type and hub, ubc mutants that lack histone H2B monoubiquitination (H2Bub1) of Arabidopsis to explore the molecular mechanisms of H2Bub1 is involved in regulating the response to salt stress. We will investigate that the role of H2Bub1 in the regulation of sensory function of microtubules cytoskeleton, and the mechanisms of tyrosine phosphorylation in the regulation the dynamics of MT in salt stress response, the function of the major targets and signaling processes for H2Bub1 participate in salt stress response. The results will provide evidence that H2Bub1 as an important modification with regulatory roles in the response to salt stress, microtubules as elements of a sensory hub that decodes salt stress-related signal signatures, and H2Bub1 play important roles to regulate PTP-related signaling pathway and then proceed to modulate the dynamics of MTs which is critical for plant salt stress tolerance. The results will provide novel insight into the mechanisms of epigenetic regulation in the response to salt stress.