前期研究发现ve1同源的棉花受体蛋白基因家族是抗黄萎病的关键基因。然而，目前只有该家族中的个别基因对2个菌系的抗性研究报道。为了系统解析这一家族各个基因的抗性谱和抗性机制，本研究拟以该家族6个基因的转基因拟南芥和本氏烟为材料，分别接种15种黄萎病菌系，确定每个基因的抗性谱。根据各个基因的特异序列构建VIGS载体，通过沉默后抗性变化进一步确定这些基因的抗病作用。将筛选到的抗菌谱广、抗性水平高的基因转化棉花。将Gbve1和Gbvdr1基因之间多个LRR区域进行替换，明确抗病蛋白识别病原菌的关键LRR结构域。最后，将这6个基因分别与GFP融合，研究该基因家族的亚细胞定位及在黄萎病侵染前后的位置变化，并检测转基因植株接种黄萎病后的活性氧、细胞凋亡和胼胝质的产生情况。本项目的实施将明确各个基因的抗性作用、阐明这些基因的抗病分子机制，获得抗性谱广、抗性水平好的基因，用于培育抗病新种质。

Previous studies found that ve1 homologous cotton receptor like genes play a key role in Verticillium resistance. However, only few genes from this family have been studied.This study will use the transgenic Arabidopsis and N. benthamiana with 6 different receptor like genes (Ve1 homologous ones) as materials, and elucidate their disease resistance by inoculating with 15 kinds of Verticillium dahliae. The resistance spectrum of each gene and the different resistance to the same strains would be elucidated. VIGS vectors were generated according to the specific sequences of each gene and each gene of the family can be silenced specifically in island cotton and the resistance of these plants will be examined to further determine the resistant function of each gene. In addition, the key LRR domain will be identified by replacing different LRR domains of Verticillium wilt resistance Gbve1 protein with non-resistance Gbvdr1, which would be important for achieving multi-resistant and broad spectrum resistance by integrating key domains of different resistance proteins. Finally, the transient expression will be carried out to determine the changes of subcellular localization after inoculation of Verticillium wilt by transforming GFP fusion genes with the six resistance one to Arabidopsis and tobacco plants. Reactive oxygen species, apoptosis and callose production will be analyzed after Verticillium wilt infection. By carrying out these experiments, the resistant function and molecular mechanisms of each gene will be identified, and the promising resistance genes may be obtained to cultivate new disease-resistant germplasm.