原位、实时、动态获取生物分子信息是探究生命现象本质的基石。成像分析是获取生物分子原位、实时信息的重要手段，是目前分析化学领域的前沿与热点研究方向。本项目以原位、实时获取活体和细胞内活性小分子成像信息为研究目标，应对荧光成像分析中，探针的生物相容性，成像稳定性和生物组织背景干扰的挑战，原创性的提出采用生物质量子点构建荧光探针，开展用于活性小分子比率荧光成像的纳米探针研究。构建具有单一波长激发双发射的比率荧光纳米探针；基于荧光共振能量转移的生物质量子点-近红外荧光染料复合物纳米探针。用于细胞和活体内活性小分子（活性氧、活性氮、活性硫和金属离子等）的识别和特异性比率荧光成像，实现对活性小分子的原位、实时、动态研究。本项目的执行将为发展高效活体成像技术提供新思路，为探究活性小分子参与的相关信号转导过程，剖析重大疾病发生和发展的分子机制以及诊疗提供新技术，对于促进生物医学研究具有重要意义。

In situ, real-time and dynamic acquisition of biomolecular information is the cornerstone of the exploration of the，essence of life phenomenon. Image analysis is an important means to obtain biological molecules in situ and real-time information, is the forward position and hot research direction in the analytical chemistry field at present. This project is to obtain active small molecular imaging information in situ and real-time as the research target to reply the challenges on the biological compatibility of probe, the imaging stability and biological tissue background interference in fluorescence imaging analysis, and proposed originally building fluorescent probes with biomass quantum dots, to carry out research for nano probe for active small molecule ratio fluorescence imaging. We will to construct the ratio fluorescent nanoprobe with single wavelength excited double emission, and biomass quantum dots-near infrared fluorescent dye complex nanoprobe based on fluorescence resonance energy transfer for the recognition and specificity of ratiometric fluorescence imaging of active small molecule (active oxygen, active nitrogen, active sulfur and metal ions) in cells and in vivo, to realize in situ, real-time and dynamic study of small active molecules. The implementation of this project will provides new ideas for the development of efficient living body imaging technology, and new technologies for researching the signal transduction process of small molecules involved in the activity and the molecular mechanism of the development, the occurrence, diagnosis and treatment of major diseases. It is of great significance to promote the research of biomedicine.