碳基量子点作为一种新型荧光纳米材料，具有荧光强度高、水溶性好、耐光漂白、低毒性、生物相容性好等优点，受到越来越多的关注。与传统的半导体荧光量子点比，碳基量子点不含重金属元素，其主要成分碳是构成生命体的元素之一，具有更低的生物毒性和更好的生物相容性。在生物医学领域显示了更大的应用前景。但目前大多数荧光碳量子点的发射光处于短波长可见光区，限制了其应用。本项目拟合成一系列掺杂碳基量子点，期望获得具有近红外和上转换发光性能，并利用掺杂碳基量子点独特的荧光性质和良好的生物兼容性，结合核酸适配体和多肽分子识别，构建一系列新型功能化荧光纳米探针，用于疾病标志物的荧光生物传感，细胞靶向特异性荧光成像和活体成像分析。本项目的实施将促进分析化学与材料科学和生物医学等相关学科的交叉融合，为疾病标志物和生物活性物质的检测及生物成像分析提供灵敏的新方法，对于推动分析化学、材料科学和生物医学的发展具有重要意义。

The carbon-based quantum dots, a new class of fluorescence nanomaterial, have attracted tremendous research interest owing to their appealing advantages such as high fluorescence intensity, superior water dispersible, high resistance to light bleaching, low toxicity and good biocompatibility. And compared with the traditional fluorescent semiconductor quantum dots, carbon based quantum dot does not contain heavy metal elements, and main component of carbon-based quantum dots is one of the element of life, and have lower toxicity and better biocompatibility. It has been found that carbon-based quantum dots are of potential as probes for high-contrast bioimaging and biosensing applications. But most fluorescent carbon-based quantum dots emitting light in the short wavelength visible light, this limits its application. The aims of this project is to synthesize a series of doped carbon quantum dots or graphene quantum dots, and expect to obtain the carbon based quantum dots with near infrared and upconversion luminescence. Using unique fluorescence properties of new type doped carbon based quantum dots and good biocompatibility, binding aptamers and polypeptides of molecular recognition to construct a series of novel functional fluorescent nanoprobes, and used as the fluorescence biosensing of disease markers, analysis of cell targeting specific fluorescence imaging and imaging in vivo. The implementation of this project will promote the cross and integration of the analytical chemistry with materials science, life science and biomedical and other related disciplines, and provide new methods for sensitive detecting disease markers and biological active substances. It is of great significance in promoting the development of analytical chemistry, life science and biomedicine.