百合是重要的商品花卉，在园林中也广泛应用。非生物逆境低温不仅影响百合商品生产，而且制约其园林应用。所以评价抗寒野生百合特异资源并挖掘抗寒基因，探究低温逆境的应答机制与调控，为百合遗传改良和栽培应用提供理论基础。参与调控植物低温胁迫应答的转录因子在百合低温应答网络中起着分子开关的作用，本研究以抗寒野生百合卷丹（Lilium lancifolium）为研究对象，对转录组测序数据分析挖掘抗寒基因并进行低温逆境的应答机制与调控研究。对RNA-seq卷丹低温胁迫基因进行系列分析，获得低温应答转录因子；采用 Gateway 技术构建卷丹低温应答关键转录因子启动子缺失体，对启动子区域顺式元件功能分析；通过诱饵表达载体构建和酵母转化，得到关键转录因子下游调控基因；利用染色质共免疫技术确定低温应答关键转录因子和下游调控基因的相互作用。最终揭示百合非生物胁迫低温逆境的应答机制与调控。

Lily is an important commodity flower, is also widely used in the garden. Abiotic stress of low temperature affects not only the lily commodity production, and its landscape application. So to evaluate cold hardiness of wild lily specific cold resistance gene resources and mining, to plore the response mechanism and regulation of adversity in low temperature, which provides the theoretical foundation for application of lily genetic improvement and cultivation. Involved in regulation of transcription factor of plant low temperature stress response in the low temperature response lily network to play the role of a molecular switch, this study cold wild tiger lily lily (Lilium lancifolium) as the research object, analysis of the transcriptome sequencing data mining cold resistance genes and the response mechanism and regulation of low temperature stress. For RNA - seq Dan gene series low temperature stress analysis, achieve low temperature response transcription factor; Gateway technology was used to construct the volume low temperature response Dan missing key transcription factor promoter, the promoter regions of cis element function analysis; Is obtained by bait expression vector to construct and yeast transformation, key transcription factors regulating downstream gene; Chromatin were immune technology is used to determine the low temperature response key transcription factors and regulating downstream gene interactions. Finally reveals lily abiotic stress response mechanism and regulation of low temperature stress.