百合是重要的球根花卉，利用种球进行无性繁殖，由于其自繁更新能力差而大大增加了百合的生产成本。LA杂种系百合‘耀眼’具有极强的自繁能力，可以在地下茎叶腋处形成数量多且质量好的茎生鳞茎，在较短的时间内达到丛生效果，快速覆盖地面，适宜多年连续生产而不需更换种球。因此，该优良性状形成机制的揭示有助于对改良百合新品种的自繁能力提供重要的理论基础和技术保证。本项目拟以LA百合‘耀眼’为研究对象，利用形态发生学、细胞生物学和分子生物学等研究手段，在亚细胞水平上明确LA百合‘耀眼’地下茎生鳞茎整个形成过程的细胞生物学变化，确定最初受到诱发因素的影响而获得再生能力的细胞位置和再生时间；探明自然环境下百合地下茎生鳞茎的启动诱发机制；探究其形成过程中生长素和细胞分裂素调控通路，揭示不同转录因子之间的相互作用以及上游顺式作用元件至下游调控基因的转录调控机制，最终阐明LA百合地下茎生鳞茎形成的分子机制。

Lily is an important bulbous flower and bulb is the asexual propagation material of lily. The production cost of lily was greatly increased because of its poor self-propagation and regeneration ability. LA hybrid lily 'Aladdin' has a strong ability of self - propagation, can form a large number of good quality bulbs at the underground stem leaf axils in a short period of time to achieve cluster effect and rapidly cover the ground, suitable for continuous production for many years without the need to change the bulbs. Therefore, the discovery of the formation mechanism of the strong self - propagation ability is helpful to provide an important theoretical basis and technical guarantee for improving the self-propagation ability of new lily varieties. In the present project, by using morphological, cytobiological and molecular biological research methods, LA hybrid lily ‘Aladdin’ was used as the research object, to explore the cellular biological changes of the whole formation process of LA hybrid lily ‘Aladdin’ underground stem axillary bulblet at subcellular level, to determine the cell position and regeneration time of the regeneration ability which was initially affected by the inducing factors; To explore the initiation and induction mechanism of lily underground stem axillary bulblet in natural environment; To explore the regulation pathway of Auxin and Cytokinin in the formation process, to reveal the interaction between different transcription factors and the transcription regulation mechanism of upstream cis-acting elements to downstream regulatory genes, and finally to clarify the molecular mechanism of the formation of LA lily underground stem axillary bulblet.