Nell-1调控Runx2/Osterix信号轴在种植体骨结合形成中的作用及机制研究

种植体粗化表面如何促进骨髓间充质干细胞成骨分化, 从而形成种植体骨结合，具体的形成机制尚不清楚。目前认识的Nell-1比BMP2更具有骨诱导作用，而Nell-1 相关机制的研究大都集中在骨质疏松、骨缺损修复等领域。Runx2和Osterix是两个关键的成骨转录因子，可调节成骨相关因子的表达。本项目提出了Nell-1可调控Runx2/Osterix的表达，并影响种植体骨结合的形成，并以此设想为基础，通过构建慢病毒过表达/干扰型载体将Nell-1基因转染进入BMSCs，探讨Nell-1调控Runx2/Osterix信号轴在种植体骨结合中的作用及机制；然后通过细胞薄层技术获得Nell-1转染的BMSCs薄层，并在钛片表面培养，体内外实验来评价该薄层在种植体骨结合中的作用及机制。本实验可为种植体骨结合机制提供新的实验依据和治疗靶点，为快速建立并长期维持种植体骨结合提供科学而合理的理论支持。

It is not clear about the mechanism how the roughened surface on dental implant promote bone marrow mesenchymal stem cells into osteoblasts to form osseointegration. Nell-1 is known having greater osteoinduction function than BMP2, and the study of Nell-1 related mechanism mostly concentrated in osteoporosis and repair of bone defect. Runx2 and Osterix are two key osteogenic transcription factors, can regulate the expression of bone related gene factors. This project presents Nell-1 can regulate the expression of Runx2/Osterix and has effect on the formation of osseointegration, and on this basis, through the construction of the overexpression and interference lentivirus vector, Nell-1 gene is transfected into BMSCs to investigate in the effect and mechanism of Nell-1 regulation of Runx2/Osterix signaling axis. Then we obtain genetically modified BMSCs cell sheet by light induced cell sheet techonology and culture on titanium surface to evaluate the effect and mechanism of the thin layer on implant osseointegration. This process can provide a new experimental basis and therapeutic target for the mechanism of implant osseointegration, to establish and maintain long-term implant osseointegration and provide scientific and reasonable theoretical support.