肝郁证作为近几年的研究热点，其形成机制及证侯内涵至今尚不完全清楚。逍遥散作为中医药调节情志疾病的经典方剂，在防治肝郁证领域具有独特的优势。临床试验证实逍遥散对肝郁证具有阻断和逆转作用，但其调控机制与药效靶标尚未阐明。基于我们前期的研究表明,肝郁证的发病与脑功能BOLD信号的紊乱有关，同时伴随肝、脑多器官色氨酸代谢微环境失衡。由此，我们设想逍遥散可通过介导肝脑多系统色氨酸代谢动态平衡，从而调控脑功能网络稳态，达到防治肝郁证的作用。本项目拟以脑功能分析和色氨酸/犬尿氨酸代谢微环境平衡作为切入点，结合静息态fMRI技术、代谢组学、基因组、转录组分析以及敲基因小鼠平台，多层次、多靶点分析肝郁证的内在本质以及逍遥散治疗机制。阐明肝郁证的发病基础及靶标调控机制，同时解答逍遥散治疗肝郁证的现代分子生物机制及作用基础，为肝郁证的有效预防及调控寻找新的分子靶点，并为药物开发提供新的思路。

As a research hotspot in recent years, the mechanismand syndrome connotation of liver stagnation syndrome have not yet been fully clear. Xiao yao San as a classic prescription for traditional chinese medicine to regulate emotional diseases, It has a unique advantage in the prevention and treatment of liver stagnation syndrome. Clinical trials have confirmed that Xiao yao San could block and reverseliver stagnation syndrome, but its regulatory mechanism and efficacy targets are still not fully elucidated. Based on our previous studies show that the incidence of liver stagnation syndromeare related to the brain functional BOLD signal disorder, accompanied by liver, brain, multi-organ tryptophan metabolism microenvironment imbalance. Thus, we hypothesis that Xiao yao San can regulate the homeostasis of brain functional network by mediating homeostasis of tryptophan metabolism in multi-systems of the liver , brain, etc. and achieve the goal of prevention and treatment of liver stagnation syndrome. The project intends to take brain functional analysis and tryptophan/ kynurenine metabolic microenvironment balance as a starting point, combined with resting state fMRI technology, metabolomics, genome, transcriptome and knockout mice platform, andinvestigate the intrinsic mechanism of liver stagnation syndrome and Xiao yao san treatment mechanism with multi-level, multi-target approach. To further clarify the pathogenesis of liver stagnation syndrome and target regulation mechanism andinvestigate the modern molecular biological mechanism and role of Xiao yao San for the treatment, we could better search for new molecular targets for the effective prevention and regulation of liver stagnation syndrome and provide new ideas for drug discoveries.