促进血管新生的水凝胶已成为再生医学和生物材料学领域关注的研究热点。基于本课题组天然多糖复合支架材料和内皮细胞钙粘素、血管内皮细胞生长因子融合蛋白的研究基础，本项目拟通过以双端巯基化蛋白酶响应性断裂多肽交联VE-cadherin-Fc/VEGF-Fc/RGD功能化透明质酸丙烯酰肼化物，构建具有蛋白酶响应性降解性能、融合蛋白/多肽联合功能化的天然多糖透明质酸水凝胶。研究水凝胶物理、化学特性及其体内外对血管内皮细胞及人源间充质干细胞的黏附、增殖、迁移、分化及血管新生的影响，揭示水凝胶降解与多组分间的相互作用及其协同作用对水凝胶网络结构和促进血管新生功能的影响机制及调控方法。开发能促进血管新生的水凝胶，并探索其在肝组织工程研究中的应用。该研究成果不仅可进一步完善生物材料生物功能化设计及制作理论，还将推动用于生物医学基础研究和再生医学临床治疗的组织工程产品的开发，具有重要的科学意义和应用价值。

Hydrogel for promoting angiogenesis have become hotspot of regenerative medicine and biomaterial. Combining natural polysaccharide scaffolds with VE-Cadherin-Fc / VEGF-Fc /RGD, in this project, a multi-component protein / peptide/polysaccharide hydrogel is designed, and used in biomimetic building of the extracellular microenvironment for promoting angiogenesis.The hydrogel is prepared by HS-ESP-miniPEG-SH cross-linked fusion protein or peptide functionalized hyaluronic acid, and characterized with the physical / chemical properties of the hydrogel and the enhancement of vascular endothelial cells / human mesenchymal stem cell adhesion, proliferation, migration ,differentiation and angionesis in vivo and in vitro. We try to reveal the regulatory mechanism of the structure of hydrogel and angiogenesis by the synergy interactions of biogradable and multicompositions of hydrogel. Optimization of the hydrogel for promoting angiogenesis will provide theoretical and technical guidance for developing novel tissue engineering scaffolds, and explore the application in liver tissue engineering research. The findings not only further improve the design and production theory of the bio-functional materials, but also promote the development of tissue engineering products used in biomedical basic research and regenerative medicine clinical treatment.