肠道微生物在昆虫的营养代谢、生长发育等关键生理过程中发挥了重要作用。但从昆虫肠道微生物与宿主互作的角度研究昆虫反寄主防御机制以及昆虫调控肠道微生物以适应寄主多样性方面的研究还非常有限。本项目以茶树重要害虫茶尺蠖及其肠道微生物为对象，应用16srDNA和宏基因组学测序技术研究茶尺蠖肠道微生物的分布和丰度，挖掘并验证其代谢有毒次生物质的功能，探明其在反防御茶叶有毒次生物质中的作用。同时比较不同寄主对茶尺蠖肠道微生物结构和功能的影响，揭示茶尺蠖调控肠道菌群适应寄主多样性的机制。该研究不仅能从昆虫-肠道微生物协同互作的角度为寄主适应机制的阐释提供新的科学依据，同时也为该害虫的综合防治提供新思路。

It has been indicated that gut microbiota involved in many vital physical processes of host including nutrients metabolizing、development and growing. However, few studies were conducted to host-plant adaptation mechanisms from the cooperation between insect and its gut microbiota and how do insect regulate its gut microbiota to accommodate the host diversity. In present study, using Ectropis obliqua Prout, one of the most important pest of tea and its gut microbiota as a research object, we will dissect the structure and function of gut microbiota in the Ectropis obliqua Prout by 16s rDNA pyrotag and metagenomics analysis, and test it. Meanwhile, we will compare the structure and function of gut microbiota of Ectropis obliqua Prout by feeding different host plant to reveal the mechanism of insect regulate its gut microbiota to adapated to host diversity. The results of these studies will not only present novel evidence to answer the mechanisms of host-plant adaptation, but also provide new thought for developing the control strategy on this pest species.