基于皮肤免疫激活与艾灸始动机制的密切关系，结合microRNA是表观遗传调节子，且microRNA155与P2X7的靶向调控在皮肤免疫激活中的重要作用，我们提出“microRNA155靶向P2X7的表观遗传调控是艾灸激活穴位皮肤免疫的关键分子机制”工作假说，并拟以足三里为穴位代表，以佐剂性关节炎动物模型为研究载体，以“microRNA155靶向P2X7的表观遗传调控”为切入点，综合运用流式细胞分选、免疫荧光多标、microRNA研究的经典技术（实时荧光定量PCR、mimics激动、inhibitor抑制、双荧光素酶miRNA靶点验证）、P2X7条件基因敲除，从正、反两个途径，揭示microRNA155-P2X7表观遗传调控在艾灸激活穴位皮肤免疫中的作用机制，为深入阐释艾灸作用的穴位局部始动机制，推动艾灸作用原理的深入研究提供丰富的科学依据。

Based on novel findings in our previous studies that both the skin immunity on acupoint would get involved in the mechanism of moxibustion therapy and P2X7 would be a key molecule at local acupoint responding to moxibustion , and combining the fact that the relationship between miR-155 and P2X7 which play important role in immune system network regulation, we propose that “microRNA155 targeting P2X7 activating local skin immune on acupoint would be able to the key initiating step of moxibustion effect”. Guided by this hypothesis, we will give moxibustion stimulation at ST36 ,establish adjuvant arthritis mice model, and apply flow cytometry, immunofluorescence multi-label techniques, miRNA research's classic technology (real-time fluorescent quantitative PCR, mimics excited, inhibitor suppression, dual luciferase miRNA target validation), conditional knockout of P2X7 receptor, and so on, to reveal the role of microRNA155-P2X7 in local skin immune activation of moxibustioned acupoints and expect to provide more evidences to underlying the initiating mechanism of moxibustion at the local acupoint.