肥大细胞脱颗粒-树突细胞迁移-T细胞免疫稳态：艾灸免疫调节核心路径研究

基于前期研究发现的肥大细胞和树突细胞是艾灸穴位免疫激活的关键参与细胞，结合肥大细胞介导的免疫调节研究前沿，提出“肥大细胞脱颗粒-树突细胞迁移-T细胞免疫稳态可能是艾灸免疫调节的核心路径”的工作假说，并拟以足三里为穴位代表，以佐剂性关节炎模型为研究载体，以肥大细胞脱颗粒-树突细胞迁移-T细胞应答轴为切入点，综合运用活体荧光标记示踪、双光子活体成像、抑制剂阻断、条件荧光标记转基因、流式细胞、免疫荧光多标等技术，在体与离体研究相结合，探寻由肥大细胞脱颗粒始动介导艾灸免疫调节的关键始动环节及其触发的核心路径，为深入阐释艾灸免疫调节机制，推动艾灸作用原理的深入研究提供丰富的科学依据，并在一定程度上为回答“针所不为，灸之所宜”的科学内涵提供线索。

Based on previous studies, we found that mast cells and dendritic cells are key cells in the local response of moxibustion acupoints. Combined with frontier research reports, we put forward the working hypothesis: mast cell degranulation-dendritic cell migration-immune homeostasis axis mediated immune regulation of moxibustion. We will take Zusanli as the representative of acupoints, take adjuvant arthritis model as research carrier, and set mast cell degranulation-dendrite migration-immune homeostasis axis theory as a research cut-in point. By using conditional gene knockout, flow cytometry, immunofluorescence multi-label and two-photon in vivo imaging techniques, we intend to reveal the mechanism of mast cell degranulation initiated the immune-regulation function of moxibustion with gain- and loss-of-function strategies. The purpose of this study is to elucidate the local initiation mechanism of moxibustion and the overall regulation mechanism of immunity. The research will also provide more scientific basis to understand the mechanism of moxibustion, and it can promote the understanding of in-depth study of the principle of action of moxibustion provide more evidence to answer the scientific connotation of "what acupuncture do not do, what moxibustion is appropriate".