效应蛋白（effector protein）是病原菌重要的致病因子，可通过诱导植物细胞坏死从而有利于死体营养型（necrotrophic）病原菌从寄主植物吸收营养。由死体营养型真菌指状青霉（Penicillium digitatum）引起的绿霉病是柑橘采后主要的病害，然而目前对该病原菌的致病机制了解甚少。前期我们已经成功鉴定到一个诱导植物细胞坏死的指状青霉效应蛋白PdE1，并初步证实PdE1可能在指状青霉侵染柑橘果实过程中发挥重要作用。本项目拟解析PdE1在指状青霉致病性中的作用，明确PdE1的分泌和时空特征，鉴定与PdE1互作的柑橘果实靶标蛋白，明确互作的位点和方式以及靶标蛋白在细胞坏死和果实抵抗病原菌侵染中的作用，最终阐明PdE1调控柑橘绿霉病菌致病的分子机理。研究结果对揭示柑橘绿霉病菌的致病机制以及效应蛋白在采后病原真菌中的作用具有重要意义，也为柑橘采后病害的防治奠定理论基础。

As an important pathogenicity factor, effector proteins from necrotrophic pathogens can induce plant cell death which is a virulence mechanism employed by necrotrophic pathogens to more readily absorb nutrients from host plants. Green mold, caused by the necrotrophic fungus Penicillium digitatum (Pd), is the major postharvest disease of citrus fruit, but little is currently known about Pd pathogenesis on citrus fruit. In our previous work, we obtained a plant cell death-inducing effector protein PdE1 (P. digitatum effector protein 1) from Pd, and preliminarily confirmed that PdE1 plays an important role in Pd infection on citrus fruit. In this project, we will investigate the role of PdE1 in Pd pathogenicity, define the secretory and temporal-spatial characteristics of PdE1, identify the interacting protein (target protein) of PdE1 from citrus fruit, define the site and mode of interacting between PdE1 and target protein, investigate the role of target protein in plant cell death and fruit resistance to pathogens, and finally elucidate the molecular mechanism of effector PdE1 to regulate Pd infection on citrus fruit. The results will be of great significance to elucidate Pd pathogenesis on citrus fruit and the role of effector proteins in postharvest pathogenic fungi, and also provide theoretical basis for controlling postharvest diseases of citrus fruit.